Biology
12th Edition
ISBN: 9780134813448
Author: Audesirk, Teresa, Gerald, Byers, Bruce E.
Publisher: Pearson,
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Chapter 27, Problem 5MC
Summary Introduction
Introduction: A growth curve represents the population growth at each period of time. The growth curve can be demonstrated by two types of curve, such as J-shaped growth curve and sigmoid growth curve. J-shaped growth curve represent the unrestricted or exponential population growth, while sigmoid growth curve represents the slow or restricted growth of population.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 27 Solutions
Biology
Ch. 27.1 - Prob. 1CSCCh. 27.1 - Prob. 1HYEWCh. 27.1 - The Return of the Elephant Seals Female elephant...Ch. 27.1 - Prob. 1CYLCh. 27.1 - describe how the growth rate interacts with...Ch. 27.1 - Prob. 3CYLCh. 27.2 - What factors might make these population data...Ch. 27.2 - Prob. 2TCCh. 27.2 - What benefits does mass emigration give to animals...Ch. 27.2 - Prob. 1CSC
Ch. 27.2 - Prob. 1CYLCh. 27.2 - Prob. 2CYLCh. 27.2 - Prob. 3CYLCh. 27.3 - Prob. 1CYLCh. 27.3 - Prob. 2CYLCh. 27.3 - Prob. 3CYLCh. 27.4 - Prob. 1CYLCh. 27.4 - Prob. 1CTCh. 27.5 - Prob. 1TCCh. 27.5 - Prob. 2TCCh. 27.5 - Prob. 3TCCh. 27.5 - describe the advances that have allowed...Ch. 27.5 - explain why rapid population growth continues...Ch. 27.5 - Prob. 3CYLCh. 27.5 - Prob. 4CYLCh. 27 - Prob. 1MCCh. 27 - Prob. 2MCCh. 27 - Prob. 3MCCh. 27 - Prob. 4MCCh. 27 - Prob. 5MCCh. 27 - Prob. 1FIBCh. 27 - The type of growth that occurs in a population...Ch. 27 - Prob. 3FIBCh. 27 - The type of spatial distribution likely to occur...Ch. 27 - Prob. 5FIBCh. 27 - Prob. 1RQCh. 27 - Prob. 2RQCh. 27 - Draw, name, and describe the properties of a...Ch. 27 - Prob. 4RQCh. 27 - What is logistic population growth? What is K?Ch. 27 - Prob. 6RQCh. 27 - Distinguish between populations showing concave...Ch. 27 - Prob. 8RQCh. 27 - Prob. 9RQCh. 27 - Prob. 1ACCh. 27 - Prob. 2AC
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