Biochemistry (Looseleaf)
Biochemistry (Looseleaf)
9th Edition
ISBN: 9781319114800
Author: BERG
Publisher: MAC HIGHER
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Chapter 27, Problem 18P
Interpretation Introduction

(a)

Interpretation:

RQ value for glucose oxidation and tripalmitoylglycerol should be calculated.

Concept introduction:

RQ (respiratory quotient) value is the ratio of the volume of carbon dioxide produced to the amount of oxygen gas required in respiration process over a fixed period of time. Its value can be zero, less than one, one, and more than one.

Interpretation Introduction

(b)

Interpretation:

The use of RQ measurements of different energy sources during intense exercise should be determined.

Concept introduction:

RQ (respiratory quotient) value is the ratio of the volume of carbon dioxide produced to the amount of oxygen gas required in the respiration process over a fixed period of time. Its value can be zero, less than one, one, and more than one.

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Fura-2 Fluorescence (Arbitrary Unit) 4500 4000 3500 3000 2500 2000 1500 1000 500 [Ca2+]=2970nM, 25°C [Ca2+] 2970nM, 4°C [Ca2+]=0.9nM, 25°C [Ca2+] = 0.9nM, 4°C 0 260 280 300 340 360 380 400 420 440 Wavelength (nm) ← < The figure on the LHS shows the excitation spectra of Fura-2 (Em = 510 nm) in 2 solutions with two different Ca2+ ion concentration as indicated. Except for temperature, the setting for excitation & signal acquisition was identical.< ப a) The unit in Y-axis is arbitrary (unspecified). Why? < < b) Compare & contrast the excitation wavelength of the Isosbestic Point of Fura-2 at 25 °C & 4 °C. Give a possible reason for the discrepancy. < c) The fluorescence intensity at 25 °C & 4 °C are different. Explain why with the concept of electronic configuration. <
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