The features of tRNA Ala which are recognized by Ala-tRNA synthetase. Introduction : RNA (ribonucleic acid) is a nucleic acid and acts as the genetic material in viruses. It performs various other functions such as coding, decoding, expression of genes, and regulation. One of the types of RNA is tRNA (transfer RNA).

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Answer 1

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Chapter 27, Problem 14P

(a)

Summary Introduction

To explain: The features of tRNA^Ala which are recognized by Ala-tRNA synthetase.

Introduction:

RNA (ribonucleic acid) is a nucleic acid and acts as the genetic material in viruses. It performs various other functions such as coding, decoding, expression of genes, and regulation. One of the types of RNA is tRNA (transfer RNA).

(b)

Summary Introduction

To describe: Consequence of C→G mutation in third position of anticodon of tRNA^Ala.

Introduction:

There are twenty amino acids which are coded by 64 codons. So it is obvious that there are certain amino acids which are coded by more than one codon. This property is known as degeneracy of codons.

(c)

Summary Introduction

To describe: Other kinds of mutations that would result in insertion of proline in place of alanine.

Introduction:

Mutation is the alteration in the sequence of the nucleotide of genome of extrachromosomal DNA, virus or an organism. Mutations can be insertion, deletion or substitution of one or more nucleotide base. In substitution mutation, single nucleotide is exchanged for another nucleotide.

(d)

Summary Introduction

To describe: The reason behind the absence of this type of mutation in natural population.

Introduction:

Mutation is the alteration in the sequence of the nucleotide of genome of extrachromosomal DNA, virus or an organism. Mutations can be insertion, deletion or substitution of one or more nucleotide base. In substitution mutation, single nucleotide is exchanged for another nucleotide.

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Draw a diagram to demonstrate 3' to 5' exonuclease activity?

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You were tasked with purifying an untagged transcription factor (molecular weight 65,000Da, isoelectric point unknown) from a contaminant Protein A (molecular weight 50,000 Da, isoelectric point 5.0). You were also instructed to use the protein for crystallization studies after purification.i) In the initial purification step, which type of chromatography should you attempt? Explain your choice and specify the requirements of the buffer solution you should use. ii) Analyzing the proteins recovered from step i) using SDS-PAGE, you still observed a faint band of Protein A in addition to the transcription factor band. Given the limited time available for further purification, you must choose ONE appropriate chromatography method to maximize the chances of separating the two proteins as well as your crystallization studies. Provide a detailed explanation of your selection and the techniques/strategies you would employ to achieve this.

Answer 2

Question

Chapter 27, Problem 14P

(a)

Summary Introduction

To explain: The features of tRNA^Ala which are recognized by Ala-tRNA synthetase.

Introduction:

RNA (ribonucleic acid) is a nucleic acid and acts as the genetic material in viruses. It performs various other functions such as coding, decoding, expression of genes, and regulation. One of the types of RNA is tRNA (transfer RNA).

(b)

Summary Introduction

To describe: Consequence of C→G mutation in third position of anticodon of tRNA^Ala.

Introduction:

There are twenty amino acids which are coded by 64 codons. So it is obvious that there are certain amino acids which are coded by more than one codon. This property is known as degeneracy of codons.

(c)

Summary Introduction

To describe: Other kinds of mutations that would result in insertion of proline in place of alanine.

Introduction:

Mutation is the alteration in the sequence of the nucleotide of genome of extrachromosomal DNA, virus or an organism. Mutations can be insertion, deletion or substitution of one or more nucleotide base. In substitution mutation, single nucleotide is exchanged for another nucleotide.

(d)

Summary Introduction

To describe: The reason behind the absence of this type of mutation in natural population.

Introduction:

Mutation is the alteration in the sequence of the nucleotide of genome of extrachromosomal DNA, virus or an organism. Mutations can be insertion, deletion or substitution of one or more nucleotide base. In substitution mutation, single nucleotide is exchanged for another nucleotide.

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Answer 3

Question

Chapter 27, Problem 14P

(a)

Summary Introduction

To explain: The features of tRNA^Ala which are recognized by Ala-tRNA synthetase.

Introduction:

RNA (ribonucleic acid) is a nucleic acid and acts as the genetic material in viruses. It performs various other functions such as coding, decoding, expression of genes, and regulation. One of the types of RNA is tRNA (transfer RNA).

(b)

Summary Introduction

To describe: Consequence of C→G mutation in third position of anticodon of tRNA^Ala.

Introduction:

There are twenty amino acids which are coded by 64 codons. So it is obvious that there are certain amino acids which are coded by more than one codon. This property is known as degeneracy of codons.

(c)

Summary Introduction

To describe: Other kinds of mutations that would result in insertion of proline in place of alanine.

Introduction:

Mutation is the alteration in the sequence of the nucleotide of genome of extrachromosomal DNA, virus or an organism. Mutations can be insertion, deletion or substitution of one or more nucleotide base. In substitution mutation, single nucleotide is exchanged for another nucleotide.

(d)

Summary Introduction

To describe: The reason behind the absence of this type of mutation in natural population.

Introduction:

Mutation is the alteration in the sequence of the nucleotide of genome of extrachromosomal DNA, virus or an organism. Mutations can be insertion, deletion or substitution of one or more nucleotide base. In substitution mutation, single nucleotide is exchanged for another nucleotide.

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Answer 4

Question

Chapter 27, Problem 14P

(a)

Summary Introduction

To explain: The features of tRNA^Ala which are recognized by Ala-tRNA synthetase.

Introduction:

RNA (ribonucleic acid) is a nucleic acid and acts as the genetic material in viruses. It performs various other functions such as coding, decoding, expression of genes, and regulation. One of the types of RNA is tRNA (transfer RNA).

(b)

Summary Introduction

To describe: Consequence of C→G mutation in third position of anticodon of tRNA^Ala.

Introduction:

There are twenty amino acids which are coded by 64 codons. So it is obvious that there are certain amino acids which are coded by more than one codon. This property is known as degeneracy of codons.

(c)

Summary Introduction

To describe: Other kinds of mutations that would result in insertion of proline in place of alanine.

Introduction:

Mutation is the alteration in the sequence of the nucleotide of genome of extrachromosomal DNA, virus or an organism. Mutations can be insertion, deletion or substitution of one or more nucleotide base. In substitution mutation, single nucleotide is exchanged for another nucleotide.

(d)

Summary Introduction

To describe: The reason behind the absence of this type of mutation in natural population.

Introduction:

Mutation is the alteration in the sequence of the nucleotide of genome of extrachromosomal DNA, virus or an organism. Mutations can be insertion, deletion or substitution of one or more nucleotide base. In substitution mutation, single nucleotide is exchanged for another nucleotide.

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Answer 5

Chapter 27, Problem 14P

(a)

Summary Introduction

To explain: The features of tRNA^Ala which are recognized by Ala-tRNA synthetase.

Introduction:

RNA (ribonucleic acid) is a nucleic acid and acts as the genetic material in viruses. It performs various other functions such as coding, decoding, expression of genes, and regulation. One of the types of RNA is tRNA (transfer RNA).

(b)

Summary Introduction

To describe: Consequence of C→G mutation in third position of anticodon of tRNA^Ala.

Introduction:

There are twenty amino acids which are coded by 64 codons. So it is obvious that there are certain amino acids which are coded by more than one codon. This property is known as degeneracy of codons.

(c)

Summary Introduction

To describe: Other kinds of mutations that would result in insertion of proline in place of alanine.

Introduction:

Mutation is the alteration in the sequence of the nucleotide of genome of extrachromosomal DNA, virus or an organism. Mutations can be insertion, deletion or substitution of one or more nucleotide base. In substitution mutation, single nucleotide is exchanged for another nucleotide.

(d)

Summary Introduction

To describe: The reason behind the absence of this type of mutation in natural population.

Introduction:

Mutation is the alteration in the sequence of the nucleotide of genome of extrachromosomal DNA, virus or an organism. Mutations can be insertion, deletion or substitution of one or more nucleotide base. In substitution mutation, single nucleotide is exchanged for another nucleotide.

Answer 6

Chapter 27, Problem 14P

(a)

Summary Introduction

To explain: The features of tRNA^Ala which are recognized by Ala-tRNA synthetase.

Introduction:

RNA (ribonucleic acid) is a nucleic acid and acts as the genetic material in viruses. It performs various other functions such as coding, decoding, expression of genes, and regulation. One of the types of RNA is tRNA (transfer RNA).

Answer 7

Chapter 27, Problem 14P

(a)

Summary Introduction

To explain: The features of tRNA^Ala which are recognized by Ala-tRNA synthetase.

Introduction:

RNA (ribonucleic acid) is a nucleic acid and acts as the genetic material in viruses. It performs various other functions such as coding, decoding, expression of genes, and regulation. One of the types of RNA is tRNA (transfer RNA).

Answer 8

(b)

Summary Introduction

To describe: Consequence of C→G mutation in third position of anticodon of tRNA^Ala.

Introduction:

There are twenty amino acids which are coded by 64 codons. So it is obvious that there are certain amino acids which are coded by more than one codon. This property is known as degeneracy of codons.

Answer 9

(b)

Summary Introduction

To describe: Consequence of C→G mutation in third position of anticodon of tRNA^Ala.

Introduction:

There are twenty amino acids which are coded by 64 codons. So it is obvious that there are certain amino acids which are coded by more than one codon. This property is known as degeneracy of codons.

Answer 10

(c)

Summary Introduction

To describe: Other kinds of mutations that would result in insertion of proline in place of alanine.

Introduction:

Mutation is the alteration in the sequence of the nucleotide of genome of extrachromosomal DNA, virus or an organism. Mutations can be insertion, deletion or substitution of one or more nucleotide base. In substitution mutation, single nucleotide is exchanged for another nucleotide.

Answer 11

(c)

Summary Introduction

To describe: Other kinds of mutations that would result in insertion of proline in place of alanine.

Introduction:

Mutation is the alteration in the sequence of the nucleotide of genome of extrachromosomal DNA, virus or an organism. Mutations can be insertion, deletion or substitution of one or more nucleotide base. In substitution mutation, single nucleotide is exchanged for another nucleotide.

Answer 12

(d)

Summary Introduction

To describe: The reason behind the absence of this type of mutation in natural population.

Introduction:

Mutation is the alteration in the sequence of the nucleotide of genome of extrachromosomal DNA, virus or an organism. Mutations can be insertion, deletion or substitution of one or more nucleotide base. In substitution mutation, single nucleotide is exchanged for another nucleotide.

Answer 13

(d)

Summary Introduction

To describe: The reason behind the absence of this type of mutation in natural population.

Introduction:

Mutation is the alteration in the sequence of the nucleotide of genome of extrachromosomal DNA, virus or an organism. Mutations can be insertion, deletion or substitution of one or more nucleotide base. In substitution mutation, single nucleotide is exchanged for another nucleotide.

Answer 14

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Ch. 27 - Prob. 1P Ch. 27 - Prob. 2P Ch. 27 - Prob. 3P Ch. 27 - Prob. 4P Ch. 27 - Prob. 5P Ch. 27 - Prob. 6P Ch. 27 - Prob. 7P Ch. 27 - Prob. 8P Ch. 27 - Prob. 9P Ch. 27 - Prob. 10P

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To describe: Consequence of C→G mutation in third position of anticodon of tRNAAla. Introduction: There are twenty amino acids which are coded by 64 codons. So it is obvious that there are certain amino acids which are coded by more than one codon. This property is known as degeneracy of codons.

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