HUMAN ANATOMY
6th Edition
ISBN: 9781260986037
Author: SALADIN
Publisher: MCG
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Chapter 26.3, Problem 13BYGO
Answer the following question to test your understanding of the preceding section;
13. What are the stages of oogenesis? Describe all the ways in which it differs form spermatogenesis.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 26 Solutions
HUMAN ANATOMY
Ch. 26.1 - Prob. 1BYGOCh. 26.1 - Prob. 2BYGOCh. 26.1 - Answer the following question to test your...Ch. 26.1 - Answer the following question to test your...Ch. 26.2 - Would you expect to find blood capillaries in the...Ch. 26.2 - Prob. 5BYGOCh. 26.2 - Answer the following question to test your...Ch. 26.2 - Prob. 7BYGOCh. 26.2 - Prob. 8BYGOCh. 26.2 - Prob. 9BYGO
Ch. 26.2 - Prob. 10BYGOCh. 26.2 - Prob. 11BYGOCh. 26.2 - Prob. 12BYGOCh. 26.3 - Prob. 1AWYKCh. 26.3 - Answer the following question to test your...Ch. 26.3 - Prob. 14BYGOCh. 26.3 - Prob. 15BYGOCh. 26.3 - Answer the following question to test your...Ch. 26.3 - Answer the following question to test your...Ch. 26.3 - Prob. 18BYGOCh. 26.3 - Prob. 19BYGOCh. 26.3 - Prob. 20BYGOCh. 26.4 - Prob. 21BYGOCh. 26.4 - Prob. 22BYGOCh. 26.4 - Prob. 23BYGOCh. 26.4 - Prob. 24BYGOCh. 26 - Prob. 26.1.1AYLOCh. 26 - Prob. 26.1.2AYLOCh. 26 - Prob. 26.1.3AYLOCh. 26 - Prob. 26.1.4AYLOCh. 26 - Prob. 26.2.1AYLOCh. 26 - To test your knowledge, discuss the following...Ch. 26 - Prob. 26.2.3AYLOCh. 26 - Prob. 26.2.4AYLOCh. 26 - Prob. 26.2.5AYLOCh. 26 - To test your knowledge, discuss the following...Ch. 26 - Prob. 26.2.7AYLOCh. 26 - Prob. 26.2.8AYLOCh. 26 - Prob. 26.2.9AYLOCh. 26 - Prob. 26.2.10AYLOCh. 26 - Prob. 26.2.11AYLOCh. 26 - Prob. 26.2.12AYLOCh. 26 - Prob. 26.2.13AYLOCh. 26 - To test your knowledge, discuss the following...Ch. 26 - Prob. 26.2.15AYLOCh. 26 - Prob. 26.2.16AYLOCh. 26 - Prob. 26.2.17AYLOCh. 26 - Prob. 26.2.18AYLOCh. 26 - Prob. 26.2.19AYLOCh. 26 - Prob. 26.2.20AYLOCh. 26 - Prob. 26.3.1AYLOCh. 26 - To test your knowledge, discuss the following...Ch. 26 - Prob. 26.3.3AYLOCh. 26 - To test your knowledge, discuss the following...Ch. 26 - Prob. 26.3.5AYLOCh. 26 - Prob. 26.3.6AYLOCh. 26 - To test your knowledge, discuss the following...Ch. 26 - To test your knowledge, discuss the following...Ch. 26 - To test your knowledge, discuss the following...Ch. 26 - Prob. 26.3.10AYLOCh. 26 - To test your knowledge, discuss the following...Ch. 26 - Prob. 26.3.12AYLOCh. 26 - Prob. 26.3.13AYLOCh. 26 - Prob. 26.3.14AYLOCh. 26 - To test your knowledge, discuss the following...Ch. 26 - Prob. 26.4.2AYLOCh. 26 - Prob. 26.4.3AYLOCh. 26 - Prob. 26.4.4AYLOCh. 26 - Prob. 26.4.5AYLOCh. 26 - Prob. 26.4.6AYLOCh. 26 - Prob. 26.4.7AYLOCh. 26 - To test your knowledge, discuss the following...Ch. 26 - Prob. 1TYRCh. 26 - Descent of the testes includes their passage...Ch. 26 - Four spermatozoa arise from each primordial germ...Ch. 26 - Prob. 4TYRCh. 26 - Prob. 5TYRCh. 26 - Prob. 6TYRCh. 26 - The tissue lost in menstruation is a. the b. the...Ch. 26 - Prob. 8TYRCh. 26 - Prob. 9TYRCh. 26 - Prob. 10TYRCh. 26 - Prob. 11TYRCh. 26 - Prob. 12TYRCh. 26 - Prob. 13TYRCh. 26 - Prob. 14TYRCh. 26 - Prob. 15TYRCh. 26 - Prob. 16TYRCh. 26 - Prob. 17TYRCh. 26 - Prob. 18TYRCh. 26 - Prob. 19TYRCh. 26 - Prob. 20TYRCh. 26 - Prob. 1BYMVCh. 26 - Prob. 2BYMVCh. 26 - Prob. 3BYMVCh. 26 - Prob. 4BYMVCh. 26 - Prob. 5BYMVCh. 26 - Prob. 6BYMVCh. 26 - Prob. 7BYMVCh. 26 - Prob. 8BYMVCh. 26 - Prob. 9BYMVCh. 26 - Prob. 10BYMVCh. 26 - Prob. 1WWWTSCh. 26 - Prob. 2WWWTSCh. 26 - Prob. 3WWWTSCh. 26 - Prob. 4WWWTSCh. 26 - Prob. 5WWWTSCh. 26 - Prob. 6WWWTSCh. 26 - Prob. 7WWWTSCh. 26 - Prob. 8WWWTSCh. 26 - Prob. 9WWWTSCh. 26 - Prob. 10WWWTSCh. 26 - Prob. 1TYCCh. 26 - Prob. 2TYCCh. 26 - Prob. 3TYCCh. 26 - Prob. 4TYCCh. 26 - Prob. 5TYC
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