Inquiry into Life
15th Edition
ISBN: 9781259426162
Author: Sylvia S. Mader Dr., Michael Windelspecht
Publisher: McGraw-Hill Education
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Chapter 26.1, Problem 2CYP
Explain how the PCR reaction amplifies a segment of DNA.
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Explain how PCR eventually generates a discrete-sized fragment from a much longer piece of DNA.
b) Describe how DNA is digested by different restriction enzymes
c) Describe how gel electrophoresis is used to estimate the size of DNA fragments.
Explain why a positive control and negative control are included in PCR experiments.
Explain the three steps involved in each cycle of polymerase chain reaction.Why is loading dye added to the DNA sample for gel electrophoresis?
Explain the function of the following components in a PCR reaction:− Primer, dNTP, MgCl, Taq polymerase, buffer.
Chapter 26 Solutions
Inquiry into Life
Ch. 26.1 - Describe the steps in forming Recombinant DNA.Ch. 26.1 - Discuss how the polymerase chain reaction works.Ch. 26.1 - Prob. 3LOCh. 26.1 - Prob. 1QTCCh. 26.1 - Prob. 2QTCCh. 26.1 - Would you be willing to provide your DNA for a...Ch. 26.1 - Prob. 2AQTCCh. 26.1 - Prob. 3AQTCCh. 26.1 - Prob. 1CYPCh. 26.1 - Explain how the PCR reaction amplifies a segment...
Ch. 26.1 - Prob. 3CYPCh. 26.1 - Prob. 1ACh. 26.1 - Prob. 2ACh. 26.1 - The polymerase chain reaction Use RNA polymerase...Ch. 26.2 - Prob. 1LOCh. 26.2 - Prob. 2LOCh. 26.2 - Prob. 1QTCCh. 26.2 - Prob. 2QTCCh. 26.2 - Prob. 3QTCCh. 26.2 - Prob. 1CYPCh. 26.2 - Prob. 2CYPCh. 26.2 - Prob. 4ACh. 26.2 - Prob. 5ACh. 26.3 - Compare and contrast in vivo and ex vivo gene...Ch. 26.3 - Prob. 1CYPCh. 26.3 - Prob. 2CYPCh. 26.3 - Prob. 1QTCCh. 26.3 - Prob. 2QTCCh. 26.3 - Prob. 3QTCCh. 26.3 - In this process, cells are removed from the body...Ch. 26.3 - When a cloned gene is used to modify a human...Ch. 26.4 - Prob. 1LOCh. 26.4 - Prob. 2LOCh. 26.4 - Prob. 3LOCh. 26.4 - Prob. 1CYPCh. 26.4 - Explain how comparative genomics can provide...Ch. 26.4 - Prob. 3CYPCh. 26.4 - Prob. 8ACh. 26.4 - Prob. 9ACh. 26.4 - Prob. 10ACh. 26 - Prob. S25.1BYBCh. 26 - Prob. S25.2BYBCh. 26 - Prob. S25.3BYBCh. 26 - Prob. 1CSCh. 26 - What is the difference between a genetically...Ch. 26 - Prob. 3CSCh. 26 - Prob. 1TCCh. 26 - In a genomic comparison between humans and yeast,...
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- Explain why PCR generates billions of products that are all the same sizearrow_forwardDescribe the method of a PCR technique in which you can amplify fragments randomly? Briefly.arrow_forwardExplain the importance of different temperatures in PCR. Tm of forward primer= 62 °C Tm of reverse primer= 59 °C Gene size= 3 Kbarrow_forward
- Explain what restriction enzymes are, how they function and how they can be used to make recombinant DNAarrow_forwardMost PCR reactions do not use the more expensive types of DNA polymerase, which have DNA proofreading. How might this be a problem in accurately copying specific DNA sequences of the target gene?arrow_forwardOutline the steps by which the polymerase chainreaction (PCR) amplifies a specific region of a genome.arrow_forward
- In PCR, the goal is to make copies of?arrow_forwardExplain how recombinant DNA technology uses restriction enzymes (restriction endonucleases). Explain how recombinant DNA technology uses DNA ligase enzymes.arrow_forwardBoth cloning and PCR can be used for making copies of DNA. What is the advantage or limitation of one over the other?arrow_forward
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