Inquiry into Life
15th Edition
ISBN: 9781259426162
Author: Sylvia S. Mader Dr., Michael Windelspecht
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 26.3, Problem 6A
In this process, cells are removed from the body and Victoria is used to insert a gene of interest.
- DNA fingerprint
- In vivo gene therapy
- Ex vivo gene therapy
- DNA sequencing
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RECOMBINANT DNA
BRIEFLY, DESCRIBE RECOMBINANT DNA AND GIVE ONE CONCRETE EXAMPLE. EVALUATE THE SIGNIFICANCE/PRACTICAL APPLICATIONS OF THIS DNA TECHNOLOGY BY CONSIDERING ETHICAL AND MORAL IMPLICATIONS BEHIND IT.
RECOMBINANT DNA
EXAMPLE:
MODIFIED TRAIT
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RECIPIENT ORGANISM
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ALSO, WHAT ARE YOUR INSIGHTS? IN 2 TO 3 SENTENCES.
Explain how and why PCR can be used to amplify DNA. Describe the steps in the process. Be sure to address the role of Taq DNA polymerase.
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Chapter 26 Solutions
Inquiry into Life
Ch. 26.1 - Describe the steps in forming Recombinant DNA.Ch. 26.1 - Discuss how the polymerase chain reaction works.Ch. 26.1 - Prob. 3LOCh. 26.1 - Prob. 1QTCCh. 26.1 - Prob. 2QTCCh. 26.1 - Would you be willing to provide your DNA for a...Ch. 26.1 - Prob. 2AQTCCh. 26.1 - Prob. 3AQTCCh. 26.1 - Prob. 1CYPCh. 26.1 - Explain how the PCR reaction amplifies a segment...
Ch. 26.1 - Prob. 3CYPCh. 26.1 - Prob. 1ACh. 26.1 - Prob. 2ACh. 26.1 - The polymerase chain reaction Use RNA polymerase...Ch. 26.2 - Prob. 1LOCh. 26.2 - Prob. 2LOCh. 26.2 - Prob. 1QTCCh. 26.2 - Prob. 2QTCCh. 26.2 - Prob. 3QTCCh. 26.2 - Prob. 1CYPCh. 26.2 - Prob. 2CYPCh. 26.2 - Prob. 4ACh. 26.2 - Prob. 5ACh. 26.3 - Compare and contrast in vivo and ex vivo gene...Ch. 26.3 - Prob. 1CYPCh. 26.3 - Prob. 2CYPCh. 26.3 - Prob. 1QTCCh. 26.3 - Prob. 2QTCCh. 26.3 - Prob. 3QTCCh. 26.3 - In this process, cells are removed from the body...Ch. 26.3 - When a cloned gene is used to modify a human...Ch. 26.4 - Prob. 1LOCh. 26.4 - Prob. 2LOCh. 26.4 - Prob. 3LOCh. 26.4 - Prob. 1CYPCh. 26.4 - Explain how comparative genomics can provide...Ch. 26.4 - Prob. 3CYPCh. 26.4 - Prob. 8ACh. 26.4 - Prob. 9ACh. 26.4 - Prob. 10ACh. 26 - Prob. S25.1BYBCh. 26 - Prob. S25.2BYBCh. 26 - Prob. S25.3BYBCh. 26 - Prob. 1CSCh. 26 - What is the difference between a genetically...Ch. 26 - Prob. 3CSCh. 26 - Prob. 1TCCh. 26 - In a genomic comparison between humans and yeast,...
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- Briefly present experimental and practical benefits of using PCR in DNA cloning process. Give some examples of clinical applications.arrow_forwardRestriction enzymes and DNA ligase are key components when preparing cloning vectors. True or False.arrow_forwardWhich of the following protocols is BEST used for introducing exogenous DNA into a cell with a thick outer barrier (e.g. plant cell wall)? electroporation O heat-shock transformation gene gun Olipofectionarrow_forward
- Direct modification of genes may be achieved using what? (Choose one below): DNA microarray CRISPR-Cas system Hybridizationarrow_forwardWhich is a complete list of the ingredients that are essential for PCR? nucleotides, DNA template, Taq polymerase, and plasmids nucleotides, DNA template, DNA ligase, and plasmids nucleotides, DNA template, Taq polymerase, and primers restriction enzymes, DNA template, Taq polymerase, and primers nucleotides, DNA template, DNA ligase, and primersarrow_forwardGel Electrophoresis is used in many different forms to learn about DNA, RNA or proteins. Research one laboratory method or technique that uses DNA electrophoresis in order to learn more or make determinations about DNA, RNA or Proteins. Name of technique or method Brief description of what the electrophoresis results are used for in the method. Include a link to your resources Include an image of the results or technique you describearrow_forward
- Regarding STR markers used in forensic science. Tick all the correct statements: no correct statement the PCR primers used to amplify STRs are located in the repeat units PCR primers to amplify STRs are located on both sides of the repeat units the PCR primers used to amplify STRs are coupled to a fluorochrome which is essential for the detection of amplicons they are absent from the gonosomes the allelic frequencies of STR markers vary according to the ethnicity of the individuals genotyped they are present homogeneously throughout the nuclear genomearrow_forwardCompare and contrast ex vivo and in vivo gene therapy as approaches for delivering therapeutic genes.arrow_forwardCloned human embryonic stem cells would be useful for which of the following reasons: Group of answer choices They would be a useful step towards the goal of creating cloned human babies They would allow the creation of patient-specific, immunologically-matched ES lines so that patient's immune systems would not attack the stem cells they were treated with Following treatment with a restriction enzyme, they could be inserted into a useful vectorarrow_forward
- Use the word bank below to match each biotechnology with the best description. Gene Therapy DNA fingerprinting Stem Cell Therapy Genetically Modified Organisms (GMO) Cloningarrow_forwardGenetic Engineering Terms Name: Draw a line to connect each pair of boxes DNA sequencing The use of an organism, or a component of an organism or other biological system, to make a product or process. DNA cloning The sequencing, analysis, and cutting-and-pasting of DNA A technique to make many copies of a specific DNA region in vitro (in a test tube rather than anorganism) Recombinant DNA Polymerase chain reaction A technique used.to separatę DNA fragments according to their size Gel electrophoresis DNA that is assembled out of fragments from multiple sources Biotechnology A molecular biology technique that makes many identical copies of a piece of DNA, such as a gene DNA technology The process of determining the sequence of nucleotides (As, Ts, Cs, and Gs) in a piece of DNA. ww tİ6111749/enetic-engineering-temsarrow_forwardWhat molecular biology technique is used to amplify DNA from an evidence sample? Reverse dot blotting Southern hybridization Cloning Polymerase chain reaction (PCR) Capillary electrophoresisarrow_forward
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