Human Anatomy & Physiology Plus Mastering A&P with Pearson eText -- Access Card Package (2nd Edition) (What's New in Anatomy & Physiology)
2nd Edition
ISBN: 9780134702339
Author: Erin C. Amerman
Publisher: PEARSON
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Chapter 26, Problem 5CYR
Summary Introduction
Introduction:
The male reproductive system is a very complex structure. Male reproductive organs are especially responsible for the production, storage, and discharge of male gametes or sperm. It consists of a pair of testis, accessory ducts, glands, and penis. The testes are present in a closed structure called the scrotum.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 26 Solutions
Human Anatomy & Physiology Plus Mastering A&P with Pearson eText -- Access Card Package (2nd Edition) (What's New in Anatomy & Physiology)
Ch. 26.1 - 1. What are the male and female gonads? What are...Ch. 26.1 - Which mechanisms increase the genetic variability...Ch. 26.1 - Prob. 3QCCh. 26.1 - Prob. 4QCCh. 26.1 - Prob. 5QCCh. 26.1 - Prob. 6QCCh. 26.2 - Which cell type in the testes produces sperm?...Ch. 26.2 - Prob. 2QCCh. 26.2 - 3. What is the function of the epididymis? How...Ch. 26.2 - 4. Trace the pathway that sperm take from the...
Ch. 26.2 - Prob. 5QCCh. 26.2 - Prob. 6QCCh. 26.2 - Prob. 7QCCh. 26.2 - Prob. 8QCCh. 26.2 - Prob. 9QCCh. 26.2 - Which part of the duct system passes through the...Ch. 26.3 - What are the steps of spermatogenesis?Ch. 26.3 - How do sustentacular cells support developing...Ch. 26.3 - Prob. 3QCCh. 26.3 - Prob. 4QCCh. 26.3 - Prob. 5QCCh. 26.3 - On what type of cell do FSH and LH act in males,...Ch. 26.3 - 7. What are the reproductive functions of...Ch. 26.3 - Prob. 8QCCh. 26.3 - Prob. 9QCCh. 26.3 - Prob. 10QCCh. 26.3 - Prob. 11QCCh. 26.3 - Prob. 12QCCh. 26.3 - Prob. 13QCCh. 26.3 - Prob. 14QCCh. 26.4 - What are the main functions of the ovaries?Ch. 26.4 - Which three ligaments support the ovary, and to...Ch. 26.4 - What structures catch an ovulated oocyte and move...Ch. 26.4 - Prob. 4QCCh. 26.4 - Prob. 5QCCh. 26.4 - Prob. 6QCCh. 26.4 - Prob. 7QCCh. 26.4 - Prob. 8QCCh. 26.4 - Prob. 9QCCh. 26.4 - 10. How are the external genitalia of the female...Ch. 26.4 - 11. Which structures do not fully develop in the...Ch. 26.4 - Prob. 12QCCh. 26.5 - When in the life cycle of a female does oogenesis...Ch. 26.5 - When is development of an oocyte arrested, and...Ch. 26.5 - How many ova are produced at the end of oogenesis?...Ch. 26.5 - What are the seven stages of the ovarian cycle?...Ch. 26.5 - Prob. 5QCCh. 26.5 - Prob. 6QCCh. 26.5 - Prob. 7QCCh. 26.5 - Prob. 8QCCh. 26.5 - 9. How do levels of ovarian hormones and...Ch. 26.5 - What are the similarities between the male and...Ch. 26.5 - What are the differences between the male and...Ch. 26.5 - Prob. 12QCCh. 26.5 - What are the female secondary sex characteristics?Ch. 26.5 - Prob. 14QCCh. 26.5 - Prob. 15QCCh. 26.6 - 1. Why do most behavioral methods of birth...Ch. 26.6 - Prob. 2QCCh. 26.6 - How do oral contraceptive pills prevent pregnancy?Ch. 26.6 - Prob. 4QCCh. 26.6 - 5. How do intrauterine devices prevent...Ch. 26.6 - Which methods of birth control are also called...Ch. 26.7 - Prob. 1QCCh. 26.7 - Prob. 2QCCh. 26.7 - Prob. 3QCCh. 26.7 - Prob. 4QCCh. 26 - Prob. 1CYRCh. 26 - Match the specific phase of meiosis with the...Ch. 26 - Prob. 3CYRCh. 26 - Which of the following structures is the site of...Ch. 26 - Prob. 5CYRCh. 26 - Prob. 6CYRCh. 26 - Match the component of the glandular secretions...Ch. 26 - Prob. 8CYRCh. 26 - Prob. 9CYRCh. 26 - Prob. 10CYRCh. 26 - Prob. 11CYRCh. 26 - Prob. 12CYRCh. 26 - Prob. 13CYRCh. 26 - Prob. 14CYRCh. 26 - Prob. 15CYRCh. 26 - Mark the following statements about oogenesis as...Ch. 26 - Prob. 17CYRCh. 26 - 18. Number the sequence of events in the hormonal...Ch. 26 - 19. Mark the following statements about the...Ch. 26 - Prob. 20CYRCh. 26 - Prob. 21CYRCh. 26 - Prob. 22CYRCh. 26 - Prob. 1CYUCh. 26 - Prob. 2CYUCh. 26 - Prob. 3CYUCh. 26 - Explain why oral contraceptives, which...Ch. 26 - Prob. 1AYKACh. 26 - Prob. 2AYKACh. 26 - Prob. 3AYKACh. 26 - Prob. 4AYKB
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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