The tetrapeptide which will produce the same fragments when treated with chymotrypsin need to be identified. Concept introduction: Enzymatic cleavage is a useful method for identifying the sequence of amino acid residue in a large peptide. This method uses enzymes for hydrolysis of the peptide bonds. These enzymes are known as peptidases. Some of the frequently used enzyme are trypsin and chymotrypsin. Trypsin selectively hydrolyzes the peptide bond at the carboxyl side of arginine and lysine. Chymotrypsin selectively hydrolyzes the peptide bond at the carboxyl end of amino acids containing aromatic side chains. The amino acids which have aromatic side chains are phenylalanine, tyrosine and tryptophan. To identify : the sequence of tetrapeptide which gives the same two fragments when treated with chymotrypsin.

Question

Want to see the full answer?

Answer 1

Question

Chapter 25.5, Problem 32ATS

Interpretation Introduction

Interpretation:

The tetrapeptide which will produce the same fragments when treated with chymotrypsin need to be identified.

Concept introduction:

Enzymatic cleavage is a useful method for identifying the sequence of amino acid residue in a large peptide. This method uses enzymes for hydrolysis of the peptide bonds. These enzymes are known as peptidases. Some of the frequently used enzyme are trypsin and chymotrypsin.

Trypsin selectively hydrolyzes the peptide bond at the carboxyl side of arginine and lysine.

Chymotrypsin selectively hydrolyzes the peptide bond at the carboxyl end of amino acids containing aromatic side chains. The amino acids which have aromatic side chains are phenylalanine, tyrosine and tryptophan.

To identify: the sequence of tetrapeptide which gives the same two fragments when treated with chymotrypsin.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

1) Suppose 0.1 kg ice at 0°C (273K) is in 0.5kg water at 20°C (293K). What is the change in entropy of the ice as it melts at 0°? To produce the original "water gas" mixture, carbon (in a combustible form known as coke) is reacted with steam: 131.4 kJ + H20(g) + C(s) → CO(g) + H2(g) From this information and the equations in the previous problem, calculate the enthalpy for the combustion or carbon to form carbon dioxide. kindly show me how to solve this long problem. Thanks

4. An 'H-NMR of a compound is acquired. The integration for signal A is 5692 and the integration for signal B is 25614. What is the simplest whole number ratio of protons for signals A and B? (Show your work!!!) 5. Assign the carbons in the NMR below as either carbonyl, aromatic, or alkyl. 200 150 100 50 ō (ppm) 1

Speaking of composite materials, indicate the correct option:(A). Composite materials can only be: metal-polymer or polymer-polymer.(B). Composite materials can be made up of particles, but not fibers or sheets.(C). When the reinforcing particles are uniformly distributed in a composite material, there may be a greater tendency for it to have isotropic properties.(D). None of the above is correct.