ANATOMY & PHYSIOLOGY 4/E PAC 1 SEMESTER
4th Edition
ISBN: 9781265061173
Author: McKinley
Publisher: MCG CUSTOM
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Chapter 25.5, Problem 16WDYL
How are fixed acids distinguished from volatile acids?
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The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions.
1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction?
2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe:
a) What molecule do you recommend for the trial. Why?
b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for?
3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale.
4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale.
Mutation 1
Mutation 2
There is currently a H5N1 cattle outbreak in North America.
According to the CDC on Feb 26*: "A multistate outbreak of HPAI A(H5N1) bird flu in dairy cows was first reported on March 25, 2024. This is the first time that these bird flu viruses had been found in cows. In the United States, since 2022, USDA has reported HPAI A(H5N1) virus detections in more than 200 mammals."
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Mechanisms 2:
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You have a bacterial strain with the CMU operon:
a) As shown in the image below, the cmu operon encodes a peptide (Pep1), as well as a
kinase and regulator corresponding to a two-component system. The cmu operon is
activated when Pep 1 is added to the growth media.
Pep1 is a peptide that when added extracellularly leads to activation of the Cmu operon.
Pep1 cmu-kinase cmu-regulator
You also have these genetic components in other strains:
b) An alternative sigma factor, with a promoter activated by the cmu-regulator, that control a
series of multiple operons that together encode a transformasome (cellular machinery
for transformation).
c) the gene cl (a repressor).
d) the promoter X, which includes a cl binding site (and in the absence of cl is active).
e) the gene gp (encoding a green fluorescence protein).
Using the cmu operon as a starting point, and assuming you can perform cloning to rearrange
any of these genomic features, how would you use one or more of these to modify the…
Chapter 25 Solutions
ANATOMY & PHYSIOLOGY 4/E PAC 1 SEMESTER
Ch. 25.1 - Prob. 1WDYLCh. 25.1 - Which ions are more prevalent in the intracellular...Ch. 25.1 - What is the major distinction in the chemical...Ch. 25.1 - Prob. 4WDYLCh. 25.2 - What are the two major sources of fluid intake?...Ch. 25.2 - How would you distinguish fluid deficiency from...Ch. 25.2 - Prob. 7WDYLCh. 25.2 - Which of these four hormonesangiotensin II,...Ch. 25.3 - Why do electrolytes exert a greater osmotic...Ch. 25.3 - Prob. 10WDYL
Ch. 25.4 - Prob. 11WDYLCh. 25.4 - How does the homeostatic system involving ADH...Ch. 25.4 - Prob. 13WDYLCh. 25.4 - Prob. 14WDYLCh. 25.5 - What is meant by acid-base balance?Ch. 25.5 - How are fixed acids distinguished from volatile...Ch. 25.5 - How do the kidneys regulate fixed acids to help...Ch. 25.5 - Prob. 18WDYLCh. 25.5 - What are the three chemical buffering systems, and...Ch. 25.5 - Prob. 20WDYLCh. 25.6 - Prob. 21WDYLCh. 25.6 - Prob. 22WDYLCh. 25.6 - Prob. 23WDYLCh. 25.6 - Prob. 24WDYLCh. 25.6 - Prob. 25WDYLCh. 25.6 - Prob. 26WDYLCh. 25 - Prob. 1DYKBCh. 25 - _____ 2. The fluid compartment with the largest...Ch. 25 - _____3. Which of the following would result in...Ch. 25 - _____4. If an individual has decreased saliva...Ch. 25 - _____5. Which hormone decreases total body fluid,...Ch. 25 - Which of the following describes an electrolyte?...Ch. 25 - Prob. 7DYKBCh. 25 - Prob. 8DYKBCh. 25 - Which of the following is not a chemical buffer in...Ch. 25 - Prob. 10DYKBCh. 25 - List the three variables that determine the...Ch. 25 - Describe the movement of water between the...Ch. 25 - Prob. 13DYKBCh. 25 - Explain the homeostatic system involving the renin...Ch. 25 - Describe how ANP is regulated and how it opposes...Ch. 25 - Describe the functions of Na+ and how it is...Ch. 25 - Describe what occurs in the kidney to maintain...Ch. 25 - Prob. 18DYKBCh. 25 - List the three chemical buffers, and describe how...Ch. 25 - Describe respiratory acidosis and its...Ch. 25 - Maria brings her baby to the emergency room. She...Ch. 25 - Prob. 2CALCh. 25 - Prob. 3CALCh. 25 - Harold has been suffering from diabetes mellitus...Ch. 25 - Prob. 5CALCh. 25 - Morgan is a nurse at the local hospital. She...Ch. 25 - Ms. Taylor, 68 years old, has been vomiting for 2...Ch. 25 - Prob. 3CSL
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- You have identified a new species of a Gram-positive bacteria. You would like to screen their genome for all proteins that are covalently linked to the cell wall. You have annotated the genome, so that you identified all the promoters, operons, and genes sequences within the operons. Using these features, what would you screen for to identify a set of candidates for proteins covalently linked to the bacterial cell wall.arrow_forwardBelow is a diagram from a genomic locus of a bacterial genome. Each arrow represents a coding region, and the arrowheads indicate its orientation in the genome. The numbers are randomly assigned. Draw the following features on the diagram, and explain your rationale for each feature: 10 12 合會會會會長 6 a) Expected transcriptions, based on known properties of bacterial genes and operons. How many proteins are encoded in each of the transcripts? b) Location of promoters (include rationale) c) Location of transcriptional terminators (include rationale) d) Locations of Shine-Dalgarno sequences (include rationale)arrow_forwardSample excuse letter in school class for the reasons of headaches and dysmenorrhea caused by menstrual cyclearrow_forward
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