The reason why the association of RNA primer with clamp maximizes the efficiency of DNA replication rather than the association of RNA primer directly to DNA polymerase. Introduction: DNA replication is a process that takes place in every biological cell involving the coping and producing two identical copies of a cell from a parent DNA molecule. There are different types of enzymes and factors whose co-participation mediate and help the other enzymes to replicate DNA efficiently. DNA clamp is a protein complex or a fold that helps the attachment of DNA polymerase enzyme to the template strand and prevents the enzyme fall-off from the strand.

Question

Suppose the data below are obtained for an enzyme catalyzed reaction with and without the inhibitor I. (s)( mM) 0.2 0.4 0.8 1.0 2.0 4.0 V without i (mM/min) 5.0 7.5 10.0 10.7 12.5 13.6 V with I (mM/min) 3.0 5.0 7.5 8.3 10.7 12.5 Make a Lineweaver Burke plot for this data using graph paper or a spreadsheet Calculate KM and Vmax without inhibitor. What type of inhibition is observed? show graph and work 2. Give the Lineweaver Burk equation and define all the parameters. 3. When substrate concentration is much greater than Km, the rate of catalysis is almost equal to a. kcat b. none of these c. all of these d. Kd e. Vmax

Answer 1

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 25, Problem 9E

Summary Introduction

To explain: The reason why the association of RNA primer with clamp maximizes the efficiency of DNA replication rather than the association of RNA primer directly to DNA polymerase.

Introduction: DNA replication is a process that takes place in every biological cell involving the coping and producing two identical copies of a cell from a parent DNA molecule. There are different types of enzymes and factors whose co-participation mediate and help the other enzymes to replicate DNA efficiently. DNA clamp is a protein complex or a fold that helps the attachment of DNA polymerase enzyme to the template strand and prevents the enzyme fall-off from the strand.

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Suppose the data below are obtained for an enzyme catalyzed reaction with and without the inhibitor I. (s)( mM) 0.2 0.4 0.8 1.0 2.0 4.0 V without i (mM/min) 5.0 7.5 10.0 10.7 12.5 13.6 V with I (mM/min) 3.0 5.0 7.5 8.3 10.7 12.5 Make a Lineweaver Burke plot for this data using graph paper or a spreadsheet Calculate KM and Vmax without inhibitor. What type of inhibition is observed? show graph and work 2. Give the Lineweaver Burk equation and define all the parameters. 3. When substrate concentration is much greater than Km, the rate of catalysis is almost equal to a. kcat b. none of these c. all of these d. Kd e. Vmax

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Please help determine the Relative Response Ratio of my GC-MS laboratory: Laboratory: Alcohol Content in Hand Sanditizers Internal Standard: Butanol Standards of Alcohols: Methanol, Ethanol, Isopropyl, n-Propanol, Butanol Recorded Retention Times: 0.645, 0.692, 0.737, 0.853, 0.977 Formula: [ (Aanalyte / Canalyte) / (AIS / CIS) ]

Answer 2

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Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 25, Problem 9E

Summary Introduction

To explain: The reason why the association of RNA primer with clamp maximizes the efficiency of DNA replication rather than the association of RNA primer directly to DNA polymerase.

Introduction: DNA replication is a process that takes place in every biological cell involving the coping and producing two identical copies of a cell from a parent DNA molecule. There are different types of enzymes and factors whose co-participation mediate and help the other enzymes to replicate DNA efficiently. DNA clamp is a protein complex or a fold that helps the attachment of DNA polymerase enzyme to the template strand and prevents the enzyme fall-off from the strand.

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Answer 3

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Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 25, Problem 9E

Summary Introduction

To explain: The reason why the association of RNA primer with clamp maximizes the efficiency of DNA replication rather than the association of RNA primer directly to DNA polymerase.

Introduction: DNA replication is a process that takes place in every biological cell involving the coping and producing two identical copies of a cell from a parent DNA molecule. There are different types of enzymes and factors whose co-participation mediate and help the other enzymes to replicate DNA efficiently. DNA clamp is a protein complex or a fold that helps the attachment of DNA polymerase enzyme to the template strand and prevents the enzyme fall-off from the strand.

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Answer 4

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Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 25, Problem 9E

Summary Introduction