Introduction to General, Organic and Biochemistry
12th Edition
ISBN: 9780357119303
Author: Bettelheim, Frederick A., Brown, William H., Campbell, Mary K., FARRELL, Shawn O., Torres, Omar
Publisher: Cengage Learning
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Chapter 25, Problem 55P
Interpretation Introduction
Interpretation:
The working of restriction endonucleases is to be explained.
Concept introduction:
Recombinant DNA techniques starts with specific circular DNA molecules present in the cells of the bacterium. These circular DNA molecules are called plasmids. Restriction endonucleases cut them at specific locations to produce sticky ends.
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5. A solution of sucrose is fermented in a vessel until the evolution of CO2 ceases. Then, the
product solution is analyzed and found to contain, 45% ethanol; 5% acetic acid; and 15%
glycerin by weight.
If the original charge is 500 kg, evaluate;
e. The ratio of sucrose to water in the original charge (wt/wt).
f. Moles of CO2 evolved.
g. Maximum possible amount of ethanol that could be formed.
h. Conversion efficiency.
i. Per cent excess of excess reactant.
Reactions:
Inversion reaction: C12H22O11 + H2O →2C6H12O6
Fermentation reaction: C6H12O6 →→2C2H5OH + 2CO2
Formation of acetic acid and glycerin: C6H12O6 + C2H5OH + H₂O→ CH3COOH + 2C3H8O3
Show work. don't give Ai generated solution. How many carbons and hydrogens are in the structure?
13. (11pts total) Consider the arrows pointing at three different carbon-carbon bonds in the
molecule depicted below.
Bond B
2°C. +2°C. cleavage
Bond A
•CH3 + 26.← Cleavage
2°C. +
Bond C
+3°C•
CH3 2C
Cleavage
E
2°C. 26.
weakest bond
Intact molecule
Strongest 3°C 20.
Gund
Largest
argest
a. (2pts) Which bond between A-C is weakest? Which is strongest? Place answers in
appropriate boxes.
C
Weakest
bond
A
Produces
Most
Bond
Strongest
Bond
Strongest Gund
produces least stable
radicals
Weakest
Stable radical
b. (4pts) Consider the relative stability of all cleavage products that form when bonds A,
B, AND C are homolytically cleaved/broken. Hint: cleavage products of bonds A, B,
and C are all carbon radicals.
i. Which ONE cleavage product is the most stable? A condensed or bond line
representation is fine.
13°C. formed in
bound C
cleavage
ii. Which ONE cleavage product is the least stable? A condensed or bond line
representation is fine.
• CH3
methyl radical
Formed in Gund A Cleavage
c.…
Chapter 25 Solutions
Introduction to General, Organic and Biochemistry
Ch. 25.1 - Prob. 25.1QCCh. 25.2 - Problem 26-1 DNA is highly condensed in the...Ch. 25.3 - Prob. 25.3QCCh. 25.4 - Prob. 25.4QCCh. 25.5 - Prob. 25.5QCCh. 25.6 - Prob. 25.6QCCh. 25.7 - Prob. 25.7QCCh. 25.8 - Prob. 25.8QCCh. 25.9 - Prob. 25.9QCCh. 25 - Does the term gene expression refer to: (a)...
Ch. 25 - Prob. 2PCh. 25 - Where does most Of the translation occur in a...Ch. 25 - Prob. 4PCh. 25 - Prob. 5PCh. 25 - Prob. 6PCh. 25 - Prob. 7PCh. 25 - What is the function of RNA polymerase?Ch. 25 - What is the role of helicase in transcription?Ch. 25 - Prob. 10PCh. 25 - Prob. 11PCh. 25 - Prob. 12PCh. 25 - Prob. 13PCh. 25 - Prob. 14PCh. 25 - Prob. 15PCh. 25 - What are the different names used for the two...Ch. 25 - Prob. 17PCh. 25 - Prob. 18PCh. 25 - Prob. 19PCh. 25 - Prob. 20PCh. 25 - Where are the codons located?Ch. 25 - What are the two most important sites on tRNA...Ch. 25 - Prob. 23PCh. 25 - Prob. 24PCh. 25 - Prob. 25PCh. 25 - Prob. 26PCh. 25 - Prob. 27PCh. 25 - (a) If a codon is GCU, what is the anticodon? (b)...Ch. 25 - If a segment of DNA is 981 units long, how many...Ch. 25 - In what sense does the universality of the genetic...Ch. 25 - Prob. 31PCh. 25 - Prob. 32PCh. 25 - A genetic code in which two bases encode a single...Ch. 25 - What is meant by the genetic code being continuous...Ch. 25 - Prob. 35PCh. 25 - Prob. 36PCh. 25 - Prob. 37PCh. 25 - Prob. 38PCh. 25 - Prob. 39PCh. 25 - What are the stages of protein synthesis?Ch. 25 - Prob. 41PCh. 25 - Explain what happens to the fMet initially put at...Ch. 25 - Prob. 43PCh. 25 - Why is amino acid activation called the second...Ch. 25 - Prob. 45PCh. 25 - Prob. 46PCh. 25 - Prob. 47PCh. 25 - Prob. 48PCh. 25 - Prob. 49PCh. 25 - What kind of interactions exist between metal-...Ch. 25 - Prob. 51PCh. 25 - Prob. 52PCh. 25 - Prob. 53PCh. 25 - Prob. 54PCh. 25 - Prob. 55PCh. 25 - Prob. 56PCh. 25 - Prob. 57PCh. 25 - Prob. 58PCh. 25 - Prob. 59PCh. 25 - Prob. 60PCh. 25 - Prob. 61PCh. 25 - Prob. 62PCh. 25 - Prob. 63PCh. 25 - Prob. 64PCh. 25 - Prob. 65PCh. 25 - Prob. 66PCh. 25 - Prob. 67PCh. 25 - Prob. 68PCh. 25 - Prob. 69PCh. 25 - Prob. 70PCh. 25 - Prob. 71PCh. 25 - Prob. 72PCh. 25 - Prob. 73PCh. 25 - Prob. 74PCh. 25 - Prob. 75PCh. 25 - Prob. 76PCh. 25 - Prob. 77PCh. 25 - Prob. 78PCh. 25 - Prob. 79PCh. 25 - Prob. 80PCh. 25 - Prob. 81PCh. 25 - Prob. 82PCh. 25 - Prob. 83PCh. 25 - Prob. 84PCh. 25 - Prob. 85PCh. 25 - Prob. 86PCh. 25 - Prob. 87PCh. 25 - Prob. 88PCh. 25 - Prob. 89PCh. 25 - Prob. 90PCh. 25 - Prob. 91PCh. 25 - Prob. 92PCh. 25 - Prob. 93PCh. 25 - Prob. 94PCh. 25 - Prob. 95PCh. 25 - Prob. 96PCh. 25 - Is there any way to prevent a hereditary disease?...Ch. 25 - Prob. 98PCh. 25 - Prob. 99PCh. 25 - Prob. 100PCh. 25 - Glycine, alanine, and valine are classified as...Ch. 25 - Prob. 102PCh. 25 - Which polypeptide is coded for by the mRNA...Ch. 25 - -100 A new endonuclease is found. It cleaves...
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