When one organism lives into the body of another organism, it is known as endosymbiosis. The other organism is called a host cell.

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Chapter 25, Problem 4TYU

Summary Introduction

Introduction:

When one organism lives into the body of another organism, it is known as endosymbiosis. The other organism is called a host cell.

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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results? 2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab? 3) Why are the cells incubated at 42°C?

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…

Answer 2

Question

Chapter 25, Problem 4TYU

Summary Introduction

Introduction:

When one organism lives into the body of another organism, it is known as endosymbiosis. The other organism is called a host cell.

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Answer 3

Question

Chapter 25, Problem 4TYU

Summary Introduction

Introduction:

When one organism lives into the body of another organism, it is known as endosymbiosis. The other organism is called a host cell.

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Answer 4

Question

Chapter 25, Problem 4TYU

Summary Introduction

Introduction:

When one organism lives into the body of another organism, it is known as endosymbiosis. The other organism is called a host cell.

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Answer 5

Chapter 25, Problem 4TYU

Summary Introduction

Introduction:

When one organism lives into the body of another organism, it is known as endosymbiosis. The other organism is called a host cell.

Answer 6

Chapter 25, Problem 4TYU

Summary Introduction

Introduction:

When one organism lives into the body of another organism, it is known as endosymbiosis. The other organism is called a host cell.

Answer 7

Chapter 25, Problem 4TYU

Summary Introduction

Introduction:

When one organism lives into the body of another organism, it is known as endosymbiosis. The other organism is called a host cell.

Answer 8

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Answer 11

Ch. 25.1 - Describe major events in the evolution of early...Ch. 25.1 - Explain why eukaryotes are said to be combination...Ch. 25.1 - Prob. 3CC Ch. 25.2 - Summarize the evidence that choanoflagellates are...Ch. 25.2 - MAKE CONNECTlONS Describe how the origin of...Ch. 25.2 - Prob. 3CC Ch. 25.3 - Briefly describe the organisms found in each of...Ch. 25.3 - MAKE CONNECTIONS Review Figures 7.2 and 8.5...Ch. 25.3 - Prob. 3CC Ch. 25.3 - Prob. 4CC

When one organism lives into the body of another organism, it is known as endosymbiosis. The other organism is called a host cell.

Solution Summary: The author explains that the description of acquired plastids by secondary endosymbiosis is incorrectly matched with the red algae.

Concept explainers

Introduction:

When one organism lives into the body of another organism, it is known as endosymbiosis. The other organism is called a host cell.

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Chapter 25 Solutions

When one organism lives into the body of another organism, it is known as endosymbiosis. The other organism is called a host cell.

Solution Summary: The author explains that the description of acquired plastids by secondary endosymbiosis is incorrectly matched with the red algae.

Concept explainers

Introduction: When one organism lives into the body of another organism, it is known as endosymbiosis. The other organism is called a host cell.

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Chapter 25 Solutions

Introduction:

When one organism lives into the body of another organism, it is known as endosymbiosis. The other organism is called a host cell.