HUMAN ANAT.+PHYSIO.(LL)-W/MASTER.+LAB
HUMAN ANAT.+PHYSIO.(LL)-W/MASTER.+LAB
19th Edition
ISBN: 9780135193952
Author: Marieb
Publisher: PEARSON
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Chapter 25, Problem 4CCS
Summary Introduction

Case summary:

Mr. Kyle Boulard just regained his health back after suffering from a crisis of acute diabetic. He is prescribed ACE that is angiotensin-converting enzyme inhibitor and a thiazide diuretic. He is given counseling on the significance of taking his medications on time and to follow his check-up routine on time. The following tests were obtained before he was discharged:

Urine: pH 6.9, negative for ketones and glucose; a 24-hour collection of urine showed 170 mg (milligram) albumin in urine per day; BP (blood pressure): 150/95, HR-75, and body temperature are 37.2°C.

Characters in the case:

Mr. Kyle Boulard suffering from acute diabetes.

Adequate information:

Mr. Kyle just recovered from the acute crisis of diabetes, so, he is advised to follow his routine checkup on time and because of high blood pressure, his kidney is damaged.

To determine:

The main problem of Mr. Kyle and how thiazide diuretic caused this problem.

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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
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