Fundamentals of Chemical Engineering Thermodynamics (MindTap Course List)
Fundamentals of Chemical Engineering Thermodynamics (MindTap Course List)
15th Edition
ISBN: 9781285968360
Author: DAHM
Publisher: Cengage
Question
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Chapter 2.5, Problem 3E

(A)

Interpretation Introduction

Interpretation:

Phase represented by point “A”.

(B)

Interpretation Introduction

Interpretation:

Phase represented by point “B”.

(C)

Interpretation Introduction

Interpretation:

Phase represented by point “C”.

(D)

Interpretation Introduction

Interpretation:

Phase represented by point “D”.

(E)

Interpretation Introduction

Interpretation:

Phase represented by point “E”.

(F)

Interpretation Introduction

Interpretation:

Phase represented by point “F”.

(G)

Interpretation Introduction

Interpretation:

Phase represented by point “G”.

(H)

Interpretation Introduction

Interpretation:

Phase represented by point “H”.

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3) A pilot-plant Podbielniak centrifugal extractor operating at 11,400 x g (this is G₁) is capable of processing 500 mL/min of filtered fermentation broth and 125 mL/min organic solvent, giving a recovery of 95%. The rotating cylinder inside the extractor has a diameter of 20 cm and is 2.5 cm wide. You need to scale up this extraction by using a larger Podbielniak extractor that has a diameter of 91 cm and width of 91 cm and delivers 2,300 x g (G2). What flow rates (in L/min) should be used in the larger extractor to achieve the same recovery efficiency?
7) You are tasked with separating two proteins by ion exchange chromatography on a 30 cm long column with an inner diameter of 2 cm. The resin has a diameter of 100 μm and a void fraction of 0.3, and your mobile phase flows through the column at a rate of Q = 5 cm³/min. The Van Deemter coefficients A, B, and C have been determined to be 0.0228 cm, 0.0036 cm²/min, and 0.00053 min, respectively, for both proteins. Protein A elutes from the column with an average retention time of 27 min and standard deviation of 0.8 min. Protein B elutes from the column. with an average retention time of 33.8 min and standard deviation of 1.0. a) How many theoretical plates does the column contain? b) What flow rate (Q) will give you the maximum resolution? c) What is the minimum height of a theoretical plate for the system?
1 5) You are asked to design a moving bed adsorption process using two columns (see the figure below). Your feed contains 100 mg/L protein and flows through both columns at 4 m³/h. Fresh resin enters the bottom of both columns (resin does not flow from the bottom column to the top column). The maximum resin flow rate that your pumps can comfortably handle is 120 kg resin/h. Experimental data suggest that the adsorption equilibrium can be modeled as qi=4ci where qi is in g protein/kg resin and c; is in g protein/L broth. (Pay attention with units!) a) What is the lowest concentration of proteins that you could get in the effluent from column 1 (indicated by the *) in mg/L? (Hint: set up a mass balance) 0.25 , * 1 2 b) What should be the flow rate of resin (in kg/h) into the second column (B2) if your overall process needs to remove 99% of the protein?
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