MICROBIOLOGY-ACCESS >CUSTOM<
MICROBIOLOGY-ACCESS >CUSTOM<
13th Edition
ISBN: 9780135668825
Author: Tortora
Publisher: PEARSON
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Chapter 25, Problem 3CAE
Summary Introduction

Introduction:

Salmonella, a genus of gram-negative, rod-shaped, cytochrome oxidase-negative, lactose-negative, non-sporulating bacteria that is the etiological agent of Salmonella gastroenteritis or salmonellosis.

Case summary:

A person (31-year old-male) became feverish 4 days after arriving at a vacation resort in Idaho. During his stay he ate at two restaurants that were not associated with the resort. At the resort he drank soft drinks with ice, used the hot tub, and went fishing. The resort is supplied by a well that was dug 3 years ago. He went to the hospital when he developed vomiting and bloody diarrhea. Gram-negative, lactose-negative bacteria were cultured from the stool sample. The patient recovered after receiving intravenous fluids.

Characters of the case:

  • A patient (Male) – 31 years old
  • Vomiting and bloody diarrhea
  • Gram-negative, lactose-negative bacteria
  • Pathogen cultures from stool sample

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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
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