The starting percentage of acetonitrile in acetonitrile/water isocratic elution in reversed-phase separation of reaction has to be given. Concept Introduction: Reversed-phase chromatography: One of the most common mode of HPLC is the reversed-phase chromatography. In reversed-phase chromatography, the mobile phase is more polar and the stationery phase is nonpolar or weakly polar. The solute is more rapidly eluted from the column in reversed-phase chromatography as the mobile phase is polar. Less polar mobile phase (solvent) has a high mobile phase strength. This method is adequate to separate mixtures of low molecular mass. To give the starting percentage of acetonitrile in acetonitrile/water isocratic elution in reversed-phase separation of reaction

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Chapter 25, Problem 25.37P

Interpretation Introduction

Interpretation:

The starting percentage of acetonitrile in acetonitrile/water isocratic elution in reversed-phase separation of reaction has to be given.

Concept Introduction:

Reversed-phase chromatography:

One of the most common mode of HPLC is the reversed-phase chromatography. In reversed-phase chromatography, the mobile phase is more polar and the stationery phase is nonpolar or weakly polar. The solute is more rapidly eluted from the column in reversed-phase chromatography as the mobile phase is polar. Less polar mobile phase (solvent) has a high mobile phase strength.

This method is adequate to separate mixtures of low molecular mass.

To give the starting percentage of acetonitrile in acetonitrile/water isocratic elution in reversed-phase separation of reaction

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