The standard free energy of the binding of methotrexate using the given three proteins and the decrease in BE due to each mutation needs to be determined. Concept introduction: Proteins are chains of amino acid joined with polypeptide bonds. Each protein has an active site in its native functional structure. In this active site, few amino acids interact with the ligands. Mutation in these amino acids can lead to functional change of the protein.

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Chapter 25, Problem 20P

Interpretation Introduction

Interpretation:

The standard free energy of the binding of methotrexate using the given three proteins and the decrease in BE due to each mutation needs to be determined.

Concept introduction:

Proteins are chains of amino acid joined with polypeptide bonds. Each protein has an active site in its native functional structure. In this active site, few amino acids interact with the ligands. Mutation in these amino acids can lead to functional change of the protein.

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