The relative enzymatic activity of GS monomers should be predicted. Concept Introduction: Glutamine synthetase (GS) (EC six.3.1.2) is a protein that plays an important role within the microorganism. GS are dodecamers with twelve active sites between every compound. Every substance will scale back the activity of the enzyme; once all final aminoalkanoic acid metabolites are certain to GS, the activity of GS is sort of utterly restrained.

Question

9) Below, there is a representation of an SDS-PAGE gel. Assuming the samples in the MW standard have masses of: 66 kDa, 45 kDa, 36 kDa, 29 kDa, 24 kDa, 20.1 kDa, and 14.2 kDa, a) Figure 4: indicate where each of the measurements were taken and label as in II.6. figure 2 above. b) As in II.7. Table 1 above create Table 2 using the data below. Determine the r.f. values for the MW standards, plot the relative mobility versus the log of the mass for the standards, and use the best fit straight line to determine the molecular weights of the proteins in the whey, peak 1, and peak 2 lanes. (5 pts—this will be scaled up appropriately if your gel did not develop properly) dye MW Whey Peak 1 Peak 2

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Question

Chapter 25, Problem 19P

Interpretation Introduction

Interpretation:

The relative enzymatic activity of GS monomers should be predicted.

Concept Introduction:

Glutamine synthetase (GS) (EC six.3.1.2) is a protein that plays an important role within the microorganism. GS are dodecamers with twelve active sites between every compound. Every substance will scale back the activity of the enzyme; once all final aminoalkanoic acid metabolites are certain to GS, the activity of GS is sort of utterly restrained.

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