BIOLOGY
12th Edition
ISBN: 9781264839698
Author: Raven
Publisher: MCG CUSTOM
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Chapter 24.3, Problem 2LO
Summary Introduction
To determine: The reason that horizontal gene transfer can complicate evolutionary hypotheses.
Introduction: The gene transfer between the bacteria takes place through the process of transformation. The naked DNA molecule is transferred from one bacterium that is resistant to ampicillin to the bacterium that is sensitive to ampicillin. As there is no other aid like a conjugation tuber or a phage particle, the genetic material transfers directly and brings about the transformation of the bacteria
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 24 Solutions
BIOLOGY
Ch. 24.1 - Prob. 1LOCh. 24.1 - Prob. 2LOCh. 24.1 - Prob. 3LOCh. 24.2 - Prob. 1LOCh. 24.2 - Prob. 2LOCh. 24.2 - Prob. 3LOCh. 24.2 - Explain why genome size and genome number do not...Ch. 24.3 - Define the terms segmental duplication, genome...Ch. 24.3 - Prob. 2LOCh. 24.4 - Prob. 1LO
Ch. 24.4 - Prob. 2LOCh. 24.5 - Prob. 1LOCh. 24.5 - Prob. 2LOCh. 24.5 - Prob. 3LOCh. 24 - Prob. 1DACh. 24 - Prob. 1IQCh. 24 - Prob. 2IQCh. 24 - Prob. 3IQCh. 24 - Humans and pufferfish diverged from a common...Ch. 24 - Prob. 2UCh. 24 - Prob. 3UCh. 24 - Homologous genes in distantly related organisms...Ch. 24 - All of the following are believed to contribute to...Ch. 24 - What is the fate of most duplicated genes? a. Gene...Ch. 24 - Chimp and human DNA whole-genome sequences differ...Ch. 24 - Prob. 2ACh. 24 - Analyze the conclusion that the Medicago...Ch. 24 - Analyze why an herbicide that targets the...Ch. 24 - The FOX2 gene is associated with speech in human....Ch. 24 - One of the common misconceptions about sequencing...Ch. 24 - How does horizontal gene transfer (HGT) complicate...
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