The reaction showing 2,4-dinitrofluorobenzene which could be used to identify the n-terminal amino acid of VAG is to be written. Further, the product of hydrolysis, when VKG is treated with 2,4-dinitrofluorobenzene, is to be identified. Concept introduction: The Sanger N-Terminal analysis is a method for sequence analysis of amino acid using 2,4-dinitrofluorobenzene (DNFB). The nucleophilic aromatic substitution reaction occurs when a polypeptide and DNFB react in mildly basic solution. The subsequent hydrolysis gives a mixture of amino acids with the N-terminal amino acid labeled with a 2,4-dinitrophenyl group.

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Chapter 24, Problem 6PP

Interpretation Introduction

Interpretation:

The reaction showing 2,4-dinitrofluorobenzene which could be used to identify the n-terminal amino acid of VAG is to be written. Further, the product of hydrolysis, when VKG is treated with 2,4-dinitrofluorobenzene, is to be identified.

Concept introduction:

The Sanger N-Terminal analysis is a method for sequence analysis of amino acid using 2,4-dinitrofluorobenzene (DNFB). The nucleophilic aromatic substitution reaction occurs when a polypeptide and DNFB react in mildly basic solution. The subsequent hydrolysis gives a mixture of amino acids with the N-terminal amino acid labeled with a 2,4-dinitrophenyl group.

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