Nester's Microbiology: A Human Perspective
Nester's Microbiology: A Human Perspective
8th Edition
ISBN: 9780073522593
Author: Denise G. Anderson Lecturer, Sarah Salm, Deborah Allen
Publisher: McGraw-Hill Education
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Chapter 24, Problem 3SA
Summary Introduction

To review:

The working of Helicobacter pylori in causing stomach ulcers.

Introduction:

Helicobacter pylori is a Gram-negative and microaerophilic bacteria, which is found usually in the stomach of the individual. It causes chronic gastritis and ulcers in the stomach. The researchers have developed a link between this bacteria and duodenal ulcers and stomach cancer.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
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