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Concept explainers
Interpretation:
Explain how the average chain growth rate of ribonucleic acid (RNA) is determined experimentally from transcription in vivo.
Concept introduction:
Transcription is defined as the process of
Also, the chain growth rate is defined as number of nucleotides added to Ribonucleic acid (RNA) segment every minute.
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Answer to Problem 1P
Solution:
The average chain growth rate can be experimentally determined in vivo by dividing the total number of
Explanation of Solution
Label the ribonucleic acid (RNA) segment as isolated and radioactive. Now, treat this segment with a mild alkali. This results in the formation of one nucleoside at the
Thus, average chain growth can be calculated by dividing the total number of nucleotide that are labelled by the total number of nucleosides formed.
Thus, it is possible to experimentally calculate the average chain growth rate from transcription in vivo.
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Chapter 24 Solutions
Biochemistry: Concepts and Connections (2nd Edition)
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