Biochemistry: Concepts and Connections (2nd Edition)
Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 24, Problem 1P
Interpretation Introduction

Interpretation:

Explain how the average chain growth rate of ribonucleic acid (RNA) is determined experimentally from transcription in vivo.

Concept introduction:

Transcription is defined as the process of polymerization of deoxyribonucleic acid (DNA) segment, in which DNA segment is copied into Ribonucleic acid (RNA) segment.

Also, the chain growth rate is defined as number of nucleotides added to Ribonucleic acid (RNA) segment every minute.

Expert Solution & Answer
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Answer to Problem 1P

Solution:

The average chain growth rate can be experimentally determined in vivo by dividing the total number of nucleotide that are labelled by the total number of nucleosides formed.

Explanation of Solution

Label the ribonucleic acid (RNA) segment as isolated and radioactive. Now, treat this segment with a mild alkali. This results in the formation of one nucleoside at the 3' terminal of RNA segment.

Thus, average chain growth can be calculated by dividing the total number of nucleotide that are labelled by the total number of nucleosides formed.

Conclusion

Thus, it is possible to experimentally calculate the average chain growth rate from transcription in vivo.

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