Human Physiology: An Integrated Approach (8th Edition)
8th Edition
ISBN: 9780134605197
Author: Dee Unglaub Silverthorn
Publisher: PEARSON
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Chapter 23.3, Problem 10CC
Summary Introduction
To determine: The development of tolerance to heat due to increased production of thyroid hormone.
Introduction: The thyroid is a butterfly-shaped endocrine gland ventrally located in the neck region. It releases the thyroid hormones, which is important for different physiological activities like maintaining basal
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 23 Solutions
Human Physiology: An Integrated Approach (8th Edition)
Ch. 23.2 - Prob. 1CCCh. 23.2 - Prob. 2CCCh. 23.2 - What do the abbreviations HPA and CBG stand for?...Ch. 23.2 - Prob. 4CCCh. 23.2 - Prob. 5CCCh. 23.2 - Prob. 6CCCh. 23.2 - Would someone with Addisons disease have normal,...Ch. 23.2 - Prob. 8CCCh. 23.3 - A woman who had her thyroid gland removed because...Ch. 23.3 - Prob. 10CC
Ch. 23.4 - Prob. 11CCCh. 23.5 - Prob. 12CCCh. 23.5 - Prob. 13CCCh. 23.6 - Prob. 14CCCh. 23.6 - Prob. 15CCCh. 23.6 - Prob. 16CCCh. 23.6 - Prob. 17CCCh. 23 - Name the zones of the adrenal cortex and the...Ch. 23 - For (a) cortisol, (b) growth hormone, (c)...Ch. 23 - Prob. 3RQCh. 23 - Prob. 4RQCh. 23 - Define each of the following terms and explain its...Ch. 23 - Prob. 6RQCh. 23 - Prob. 7RQCh. 23 - Prob. 8RQCh. 23 - Define, compare, and contrast or relate the terms...Ch. 23 - Prob. 10RQCh. 23 - Prob. 11RQCh. 23 - Osteoclasts make acid (H+) from CO2 and H2O. They...Ch. 23 - Prob. 13RQCh. 23 - One diagnostic test to determine the cause of...Ch. 23 - Prob. 15RQCh. 23 - Prob. 16RQCh. 23 - Prob. 17RQCh. 23 - Prob. 18RQCh. 23 - Prob. 19RQ
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