Human Anatomy (8th Edition)
8th Edition
ISBN: 9780134243818
Author: Elaine N. Marieb, Patricia Brady Wilhelm, Jon B. Mallatt
Publisher: PEARSON
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Question
Chapter 23, Problem 5CRCAQ
Summary Introduction
To review:
The device known as endoscope.
Introduction:
The large intestine is segmented with many parts from the cecum to the rectum. The colon is made up of many sub-segments. Various parts of the large intestine are susceptible to cancer due to the presence of various carcinogens in feces. Detection of the same requires specialized medical equipment that can detect precancerous growth. This is essential to initiate the treatment for cancer.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 23 Solutions
Human Anatomy (8th Edition)
Ch. 23 - Where in the alimentary canal does propulsion...Ch. 23 - Differentiate the abdominal cavity from the...Ch. 23 - Identify all the mesenteries that connect to each...Ch. 23 - Prob. 4CYUCh. 23 - Name the three sublayers of the mucosa. Which...Ch. 23 - Name the tissue layer of the alimentary canal that...Ch. 23 - Contrast smooth muscle to skeletal muscle, noting...Ch. 23 - What type of epithelium forms the mucosa lining...Ch. 23 - Name the three major salivary glands. What...Ch. 23 - Prob. 10CYU
Ch. 23 - How does the epithelial lining change from the...Ch. 23 - Describe the location of the stomach in reference...Ch. 23 - What do chief cells produce? What do parietal...Ch. 23 - Prob. 14CYUCh. 23 - Name all the parts of the large intestine,...Ch. 23 - Name the structures within the villus that receive...Ch. 23 - Name the vessels and ducts that pass through the...Ch. 23 - Trace the path of blood drained from the digestive...Ch. 23 - Prob. 19CYUCh. 23 - Why is it so important to wash your hands after...Ch. 23 - Which embryonic germ layer forms the epithelium of...Ch. 23 - What blood vessel supplies the structures derived...Ch. 23 - Which of the following organs is secondarily...Ch. 23 - Prob. 2RQCh. 23 - For each organ in the left-hand column, select the...Ch. 23 - Match each of the mesenteries in the key with the...Ch. 23 - Prob. 5RQCh. 23 - Prob. 6RQCh. 23 - Which of the following correctly describe the flow...Ch. 23 - The exocrine glands in the pancreas are (a) simple...Ch. 23 - Prob. 9RQCh. 23 - Which one of the following features is shared by...Ch. 23 - Prob. 11RQCh. 23 - Prob. 12RQCh. 23 - Protein digestion begins (a) in the mouth by...Ch. 23 - The calcified connective tissue that attaches the...Ch. 23 - Prob. 15RQCh. 23 - Prob. 16RQCh. 23 - The salivary gland that contains only serous cells...Ch. 23 - Use the key below to indicate the blood vessel...Ch. 23 - Make a simple drawing of the organs of the...Ch. 23 - Name the layers of the wall of the alimentary...Ch. 23 - (a) Write the dental formulas for both the...Ch. 23 - Bianca went on a trip to the Bahamas during spring...Ch. 23 - (a) Make a rough sketch of the Visceral surface of...Ch. 23 - Name four structural features that increase the...Ch. 23 - (a) List the three major vessels of the portal...Ch. 23 - Of the three major salivary glands, which is the...Ch. 23 - Trace the entire duct systems of the liver and...Ch. 23 - Prob. 28RQCh. 23 - Prob. 29RQCh. 23 - Prob. 30RQCh. 23 - Prob. 31RQCh. 23 - This chapter describes schemes that divided the...Ch. 23 - A 21-year-old man with severe appendicitis did not...Ch. 23 - Duncan, an inquisitive 8-year-old, saw his...Ch. 23 - Eva, a middle-aged attorney, complains of a...Ch. 23 - Prob. 5CRCAQCh. 23 - The janitor who cleaned the anatomy lab had a...Ch. 23 - Explain why cancer chemotherapies that stop the...Ch. 23 - From what embryonic layer (ectoderm, mesoderm, or...Ch. 23 - Name the three major salivary glands. What...Ch. 23 - Rebound tenderness in the lower right quadrant of...
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