Human Anatomy (8th Edition)
8th Edition
ISBN: 9780134243818
Author: Elaine N. Marieb, Patricia Brady Wilhelm, Jon B. Mallatt
Publisher: PEARSON
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Chapter 23, Problem 21RQ
(a) Write the dental formulas for both the deciduous and the permanent teeth. (b) Define dental pulp, and describe its location.
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d) Since the C-peptide is cut out of proinsulin to create the final mature insulin (B-chain and A-chain) what role do you think the C-peptide might play in the biosynthesis of the mature insulin protein?
a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 23 Solutions
Human Anatomy (8th Edition)
Ch. 23 - Where in the alimentary canal does propulsion...Ch. 23 - Differentiate the abdominal cavity from the...Ch. 23 - Identify all the mesenteries that connect to each...Ch. 23 - Prob. 4CYUCh. 23 - Name the three sublayers of the mucosa. Which...Ch. 23 - Name the tissue layer of the alimentary canal that...Ch. 23 - Contrast smooth muscle to skeletal muscle, noting...Ch. 23 - What type of epithelium forms the mucosa lining...Ch. 23 - Name the three major salivary glands. What...Ch. 23 - Prob. 10CYU
Ch. 23 - How does the epithelial lining change from the...Ch. 23 - Describe the location of the stomach in reference...Ch. 23 - What do chief cells produce? What do parietal...Ch. 23 - Prob. 14CYUCh. 23 - Name all the parts of the large intestine,...Ch. 23 - Name the structures within the villus that receive...Ch. 23 - Name the vessels and ducts that pass through the...Ch. 23 - Trace the path of blood drained from the digestive...Ch. 23 - Prob. 19CYUCh. 23 - Why is it so important to wash your hands after...Ch. 23 - Which embryonic germ layer forms the epithelium of...Ch. 23 - What blood vessel supplies the structures derived...Ch. 23 - Which of the following organs is secondarily...Ch. 23 - Prob. 2RQCh. 23 - For each organ in the left-hand column, select the...Ch. 23 - Match each of the mesenteries in the key with the...Ch. 23 - Prob. 5RQCh. 23 - Prob. 6RQCh. 23 - Which of the following correctly describe the flow...Ch. 23 - The exocrine glands in the pancreas are (a) simple...Ch. 23 - Prob. 9RQCh. 23 - Which one of the following features is shared by...Ch. 23 - Prob. 11RQCh. 23 - Prob. 12RQCh. 23 - Protein digestion begins (a) in the mouth by...Ch. 23 - The calcified connective tissue that attaches the...Ch. 23 - Prob. 15RQCh. 23 - Prob. 16RQCh. 23 - The salivary gland that contains only serous cells...Ch. 23 - Use the key below to indicate the blood vessel...Ch. 23 - Make a simple drawing of the organs of the...Ch. 23 - Name the layers of the wall of the alimentary...Ch. 23 - (a) Write the dental formulas for both the...Ch. 23 - Bianca went on a trip to the Bahamas during spring...Ch. 23 - (a) Make a rough sketch of the Visceral surface of...Ch. 23 - Name four structural features that increase the...Ch. 23 - (a) List the three major vessels of the portal...Ch. 23 - Of the three major salivary glands, which is the...Ch. 23 - Trace the entire duct systems of the liver and...Ch. 23 - Prob. 28RQCh. 23 - Prob. 29RQCh. 23 - Prob. 30RQCh. 23 - Prob. 31RQCh. 23 - This chapter describes schemes that divided the...Ch. 23 - A 21-year-old man with severe appendicitis did not...Ch. 23 - Duncan, an inquisitive 8-year-old, saw his...Ch. 23 - Eva, a middle-aged attorney, complains of a...Ch. 23 - Prob. 5CRCAQCh. 23 - The janitor who cleaned the anatomy lab had a...Ch. 23 - Explain why cancer chemotherapies that stop the...Ch. 23 - From what embryonic layer (ectoderm, mesoderm, or...Ch. 23 - Name the three major salivary glands. What...Ch. 23 - Rebound tenderness in the lower right quadrant of...
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- a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates? b) Predict the growth you would expect to see on each of the following plates: – DNA ___________________________________________________________ – DNA/+Amp ______________________________________________________ +DNA/+Amp ______________________________________________________ +DNA/+Amp/+IPTG _________________________________________________arrow_forward1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids? 2) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following: Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng Final recovery volume: 0.50 mL Volume plated: 0.25 mL Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) 3) Using the equation above, calculate the transformation efficiency. 4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forward
- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forwardIn oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the reaction, OTMPD is electron acceptor O cytochrome c is the electron source oxygen is terminal electron acceptor OH2 produced is electron donorarrow_forward
- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have shipped your samples off for sequencing and are now waiting for the results. Out of curiosity (and maybe boredom...) you decide to test your culture for the Catalase and Oxidase enzymes. Upon testing your sample for catalase, you don't see any bubbles; however, you do see a color change to purple during the Oxidase test. What results can you conclude from this? O Catalase-/ Oxidase + O Catalase +/ Oxidase + Catalase + / Oxidase- O Catalase / Oxidase - O None of the abovearrow_forwardWhich of the following is not a strength of using 16S rRNA for phylogenetic analyses? OA. It's cheap OB. It's easy to do C. It can be used to identify all the way down to the strain level OD. Both A & B OE. None of the abovearrow_forwardWhy are molecular approaches important to the field of microbial taxonomy and phylogeny? Phylogenetic inferences based on molecular approaches provide the most robust analysis of microbial evolution currently available. It allows for the collection of a large and accurate dataset from many organisms Almost no fossil record was left by microbes when compared to plants and animals All of the above None of the abovearrow_forward
- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forward
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