Campbell Biology (10th Edition)
10th Edition
ISBN: 9780321775658
Author: Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert B. Jackson
Publisher: PEARSON
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Chapter 23, Problem 4TYU
There are 25 individuals in population 1, all with genotype AA, and there are 40 individuals in population 2, all with genotype aa. Assume that these populations are located for from each other and that their environmental conditions are very similar. Based on the information given here, the observed
- (A) genetic drift.
- (B) gene flow.
- (C) nonrandom mating.
- (D) directional selection.
Expert Solution & Answer
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Students have asked these similar questions
1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 23 Solutions
Campbell Biology (10th Edition)
Ch. 23.1 - Explain why genetic Variation within a population...Ch. 23.1 - Ot all the mutations that occur in a population,...Ch. 23.1 - MAKE CONNECTIONS If a population stopped...Ch. 23.2 - A population has 700 individuals, 85 of genotype...Ch. 23.2 - The frequency of allele a is 0.45 for a population...Ch. 23.2 - WHAT IF? A locus that affects susceptibility to a...Ch. 23.3 - In what sense is natural selection more...Ch. 23.3 - Distinguish genetic draft from gene flow in terms...Ch. 23.3 - WH AT IF? Suppose two plant populations exchange...Ch. 23.4 - What is the relative fitness of a sterile mule?...
Ch. 23.4 - Explain why natural selection is the only...Ch. 23.4 - Prob. 3CCCh. 23 - Typically. most of the nucleotide variabillity...Ch. 23 - Is it circular reasoning to calculate p ond q from...Ch. 23 - Would two small, geographically isolated...Ch. 23 - How might secondary sex characteristics in males...Ch. 23 - Natural selection changes allele frequencies...Ch. 23 - Prob. 2TYUCh. 23 - Prob. 3TYUCh. 23 - There are 25 individuals in population 1, all with...Ch. 23 - Prob. 5TYUCh. 23 - EVOLUTION CONNECTION Using at least two examples,...Ch. 23 - Prob. 7TYUCh. 23 - Prob. 8TYUCh. 23 - SYNTHESI2E YOUR KNOWLEDGE This kettle lake forracd...
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