How does the metal ion in carboxypeptidase A increase the enzyme’s catalytic activity

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Answer 1

Textbook Question

Chapter 23, Problem 1P

How does the metal ion in carboxypeptidase A increase the enzyme’s catalytic activity

Expert Solution & Answer

Interpretation Introduction

Interpretation:

The metal ion in carboxypetidase A increases the catalytic activity of the enzyme; this has to be explained.

Explanation of Solution

Carboxypetidase A is a digestive enzyme. It hydrolyzes the carboxyl terminal peptide bond and it prefers bulky and aliphatic residues.

Proposed mechanism for the catalytic reaction of carboxypetidase is given in figure 1.

Student's Study Guide and Solutions Manual for Organic Chemistry, Chapter 23, Problem 1P

Figure 1

The metal ion present in the carboxypeptidase A enzyme is Zinc metal.

This metal ion (Zn2+) makes the carbonyl (C=O) carbon more susceptible to nucleophilic addition, increases the nucleophilicity of water molecule by making it like a hydroxide ion, and the negative charge of the transition state is stabilized by this metal ion.

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Answer 2

Textbook Question

Chapter 23, Problem 1P

How does the metal ion in carboxypeptidase A increase the enzyme’s catalytic activity

Expert Solution & Answer

Interpretation Introduction

Interpretation:

The metal ion in carboxypetidase A increases the catalytic activity of the enzyme; this has to be explained.

Explanation of Solution

Carboxypetidase A is a digestive enzyme. It hydrolyzes the carboxyl terminal peptide bond and it prefers bulky and aliphatic residues.

Proposed mechanism for the catalytic reaction of carboxypetidase is given in figure 1.

Student's Study Guide and Solutions Manual for Organic Chemistry, Chapter 23, Problem 1P

Figure 1

The metal ion present in the carboxypeptidase A enzyme is Zinc metal.

This metal ion (Zn2+) makes the carbonyl (C=O) carbon more susceptible to nucleophilic addition, increases the nucleophilicity of water molecule by making it like a hydroxide ion, and the negative charge of the transition state is stabilized by this metal ion.

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Answer 3

Textbook Question

Chapter 23, Problem 1P

How does the metal ion in carboxypeptidase A increase the enzyme’s catalytic activity

Expert Solution & Answer

Interpretation Introduction

Interpretation:

The metal ion in carboxypetidase A increases the catalytic activity of the enzyme; this has to be explained.

Explanation of Solution

Carboxypetidase A is a digestive enzyme. It hydrolyzes the carboxyl terminal peptide bond and it prefers bulky and aliphatic residues.

Proposed mechanism for the catalytic reaction of carboxypetidase is given in figure 1.

Student's Study Guide and Solutions Manual for Organic Chemistry, Chapter 23, Problem 1P

Figure 1

The metal ion present in the carboxypeptidase A enzyme is Zinc metal.

This metal ion (Zn2+) makes the carbonyl (C=O) carbon more susceptible to nucleophilic addition, increases the nucleophilicity of water molecule by making it like a hydroxide ion, and the negative charge of the transition state is stabilized by this metal ion.

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Answer 4

Textbook Question

Chapter 23, Problem 1P

How does the metal ion in carboxypeptidase A increase the enzyme’s catalytic activity

Expert Solution & Answer

Interpretation Introduction

Interpretation:

The metal ion in carboxypetidase A increases the catalytic activity of the enzyme; this has to be explained.

Explanation of Solution

Carboxypetidase A is a digestive enzyme. It hydrolyzes the carboxyl terminal peptide bond and it prefers bulky and aliphatic residues.

Proposed mechanism for the catalytic reaction of carboxypetidase is given in figure 1.

Student's Study Guide and Solutions Manual for Organic Chemistry, Chapter 23, Problem 1P

Figure 1

The metal ion present in the carboxypeptidase A enzyme is Zinc metal.

This metal ion (Zn2+) makes the carbonyl (C=O) carbon more susceptible to nucleophilic addition, increases the nucleophilicity of water molecule by making it like a hydroxide ion, and the negative charge of the transition state is stabilized by this metal ion.

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Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Textbook Question

Answer 6

Textbook Question

Answer 7

Expert Solution & Answer

Interpretation Introduction

Interpretation:

The metal ion in carboxypetidase A increases the catalytic activity of the enzyme; this has to be explained.

Explanation of Solution

Carboxypetidase A is a digestive enzyme. It hydrolyzes the carboxyl terminal peptide bond and it prefers bulky and aliphatic residues.

Proposed mechanism for the catalytic reaction of carboxypetidase is given in figure 1.

Student's Study Guide and Solutions Manual for Organic Chemistry, Chapter 23, Problem 1P

Figure 1

The metal ion present in the carboxypeptidase A enzyme is Zinc metal.

This metal ion (Zn2+) makes the carbonyl (C=O) carbon more susceptible to nucleophilic addition, increases the nucleophilicity of water molecule by making it like a hydroxide ion, and the negative charge of the transition state is stabilized by this metal ion.

Answer 8

Expert Solution & Answer

Interpretation Introduction

Interpretation:

The metal ion in carboxypetidase A increases the catalytic activity of the enzyme; this has to be explained.

Explanation of Solution

Carboxypetidase A is a digestive enzyme. It hydrolyzes the carboxyl terminal peptide bond and it prefers bulky and aliphatic residues.

Proposed mechanism for the catalytic reaction of carboxypetidase is given in figure 1.

Student's Study Guide and Solutions Manual for Organic Chemistry, Chapter 23, Problem 1P

Figure 1

The metal ion present in the carboxypeptidase A enzyme is Zinc metal.

This metal ion (Zn2+) makes the carbonyl (C=O) carbon more susceptible to nucleophilic addition, increases the nucleophilicity of water molecule by making it like a hydroxide ion, and the negative charge of the transition state is stabilized by this metal ion.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Interpretation Introduction

Interpretation:

The metal ion in carboxypetidase A increases the catalytic activity of the enzyme; this has to be explained.

Answer 12

Explanation of Solution

Carboxypetidase A is a digestive enzyme. It hydrolyzes the carboxyl terminal peptide bond and it prefers bulky and aliphatic residues.

Proposed mechanism for the catalytic reaction of carboxypetidase is given in figure 1.

Student's Study Guide and Solutions Manual for Organic Chemistry, Chapter 23, Problem 1P

Figure 1

The metal ion present in the carboxypeptidase A enzyme is Zinc metal.

This metal ion (Zn2+) makes the carbonyl (C=O) carbon more susceptible to nucleophilic addition, increases the nucleophilicity of water molecule by making it like a hydroxide ion, and the negative charge of the transition state is stabilized by this metal ion.

Answer 13

Ch. 23.1 - Prob. 2P Ch. 23.1 - Prob. 3P Ch. 23.2 - How many conjugated double bonds are there in a....Ch. 23.2 - Instead of adding to the 4a position and...Ch. 23.2 - Prob. 7P Ch. 23.3 - Prob. 8P Ch. 23.3 - Acetolactate synthase is another TPP-requiring...Ch. 23.3 - Acetolactate synthase transfers the acyl group of...Ch. 23.3 - Prob. 12P Ch. 23.5 - Which compound is more easily decarboxylated?

How does the metal ion in carboxypeptidase A increase the enzyme’s catalytic activity

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