LSC ANATOMY & PHYSIOLOGY CONNECT ACCESS
4th Edition
ISBN: 9781264929290
Author: McKinley
Publisher: MCG CUSTOM
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Textbook Question
Chapter 23, Problem 19DYKB
Describe the relationship of PCO2 and hemoglobin percent saturation.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 23 Solutions
LSC ANATOMY & PHYSIOLOGY CONNECT ACCESS
Ch. 23.1 - Which respiratory structure is associated with the...Ch. 23.1 - Prob. 2WDYLCh. 23.1 - In what ways does the epithelium of the upper...Ch. 23.2 - What changes occur to inhaled air as it passes...Ch. 23.2 - Prob. 5WDYLCh. 23.2 - What two regions of the pharynx contain tonsils?...Ch. 23.2 - How does the larynx assist in increasing abdominal...Ch. 23.2 - What are the three unpaired cartilages in the...Ch. 23.2 - Prob. 9WDYLCh. 23.3 - What is the function of the C-shaped tracheal...
Ch. 23.3 - What are the significant structural differences...Ch. 23.3 - Which of the following respiratory structures are...Ch. 23.3 - The respiratory tract can be damaged from...Ch. 23.3 - Prob. 14WDYLCh. 23.3 - Prob. 15WDYLCh. 23.4 - Match the component of the ling with its air...Ch. 23.4 - Prob. 17WDYLCh. 23.4 - What is the function of serous fluid within the...Ch. 23.4 - Why is the intrapleural pressure normally lower...Ch. 23.5 - Prob. 20WDYLCh. 23.5 - Describe the sequence of events of quiet...Ch. 23.5 - How are larger amounts of air moved between the...Ch. 23.5 - Prob. 23WDYLCh. 23.5 - Which of the following stimuli will cause an...Ch. 23.5 - Are the skeletal muscles of breathing innervated...Ch. 23.5 - The two factors that determine airflow are the...Ch. 23.5 - A person in yoga class is encouraged to take long,...Ch. 23.5 - Prob. 28WDYLCh. 23.6 - Given the same partial pressure for oxygen and...Ch. 23.6 - How do the partial pressures of oxygen and carbon...Ch. 23.6 - Prob. 31WDYLCh. 23.6 - How do the partial pressures of oxygen and carbon...Ch. 23.7 - Why is such a small percentage (about 2%) of...Ch. 23.7 - How is the majority of carbon dioxide transported...Ch. 23.7 - How does oxygen movement occur during alveolar gas...Ch. 23.7 - How does carbon dioxide movement occur during...Ch. 23.7 - Does hemoglobin saturation increase or decrease...Ch. 23.7 - How is oxygen release from hemoglobin during...Ch. 23.8 - How does blood PO2 and PCO2 change if an...Ch. 23.8 - How does blood PO2 and PCO2 change during...Ch. 23.8 - Prob. 41WDYLCh. 23 - Prob. 1DYKBCh. 23 - Prob. 2DYKBCh. 23 - Prob. 3DYKBCh. 23 - Prob. 4DYKBCh. 23 - Prob. 5DYKBCh. 23 - Which areas of the brain contain the respiratory...Ch. 23 - Prob. 7DYKBCh. 23 - Prob. 8DYKBCh. 23 - Prob. 9DYKBCh. 23 - Prob. 10DYKBCh. 23 - Explain how the respiratory tract is organized...Ch. 23 - Describe the relationship of the visceral pleura,...Ch. 23 - List the four processes of respiration, in order,...Ch. 23 - Describe the muscles, volume changes, and pressure...Ch. 23 - Explain how additional air is moved during a...Ch. 23 - Describe bow quiet breathing is controlled by the...Ch. 23 - Explain alveolar and systemic gas exchange.Ch. 23 - List the two means by which oxygen is transported...Ch. 23 - Describe the relationship of PCO2 and hemoglobin...Ch. 23 - List the variables that increase the release of...Ch. 23 - Paramedics arrived at a car accident to find an...Ch. 23 - Use the following to answer questions 24....Ch. 23 - Use the following to answer questions 24....Ch. 23 - Use the following to answer questions 24....Ch. 23 - Prob. 5CALCh. 23 - Prob. 1CSLCh. 23 - The nerve to the sternocleidomastoid muscle was...Ch. 23 - Prob. 3CSL
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
- In oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the reaction, OTMPD is electron acceptor O cytochrome c is the electron source oxygen is terminal electron acceptor OH2 produced is electron donorarrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have shipped your samples off for sequencing and are now waiting for the results. Out of curiosity (and maybe boredom...) you decide to test your culture for the Catalase and Oxidase enzymes. Upon testing your sample for catalase, you don't see any bubbles; however, you do see a color change to purple during the Oxidase test. What results can you conclude from this? O Catalase-/ Oxidase + O Catalase +/ Oxidase + Catalase + / Oxidase- O Catalase / Oxidase - O None of the abovearrow_forwardWhich of the following is not a strength of using 16S rRNA for phylogenetic analyses? OA. It's cheap OB. It's easy to do C. It can be used to identify all the way down to the strain level OD. Both A & B OE. None of the abovearrow_forward
- Why are molecular approaches important to the field of microbial taxonomy and phylogeny? Phylogenetic inferences based on molecular approaches provide the most robust analysis of microbial evolution currently available. It allows for the collection of a large and accurate dataset from many organisms Almost no fossil record was left by microbes when compared to plants and animals All of the above None of the abovearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forward
- is often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forwardWhich of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forwardAn unrooted tree containing ten unrelated species can become rooted by adding a descendant group related to two of the species. an unrelated outgroup. O a distantly related outgroup. O a descendant related to only one of the species.arrow_forward
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