(a) Interpretation: The effect of enzyme mutation on enzyme activity with varying concentrations of carnitine must be determined. The K M and V max values of wild-type and mutant enzymes must be determined. Concept introduction: Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Question

Want to see the full answer?

Answer 1

Question

Chapter 22, Problem 57P

Interpretation Introduction

(a)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of carnitine must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(b)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of palmitoyl CoA must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(c)

Interpretation:

The enzyme that is more sensitive to malonyl CoA inhibition must be determined.

Concept introductionCarnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(d)

Interpretation:

Effect of 100 µM palmitoyl CoA, 100 µM carnitine, and malonyl CoA 10µM on mutant enzyme and wild type enzyme must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(e)

Interpretation:

Role of glutamate 3 in CPTI function should be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

Biochemistry What is the process of "transamination" in either the muscles or the liver, that involves keto acid or glutamic acid? Please explain how the steps work. Thank you!

Biochemistry Please help. Thank you What is the importance of glutamic acid in the metabolism of nitrogen from amino acids? (we know therole; it’s used to remove the nitrogen from amino acids so that the remaining carbon skeleton can bebroken down by the “usual” pathways, but what is the important, unique role that only glutamicacid/glutamate can do?)

Biochemistry Please help. Thank you When carbamyl phosphate is joined to L-ornathine, where does the energy for the reaction come from?

Answer 2

Question

Chapter 22, Problem 57P

Interpretation Introduction

(a)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of carnitine must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(b)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of palmitoyl CoA must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(c)

Interpretation:

The enzyme that is more sensitive to malonyl CoA inhibition must be determined.

Concept introductionCarnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(d)

Interpretation:

Effect of 100 µM palmitoyl CoA, 100 µM carnitine, and malonyl CoA 10µM on mutant enzyme and wild type enzyme must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(e)

Interpretation:

Role of glutamate 3 in CPTI function should be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 3

Question

Chapter 22, Problem 57P

Interpretation Introduction

(a)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of carnitine must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(b)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of palmitoyl CoA must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(c)

Interpretation:

The enzyme that is more sensitive to malonyl CoA inhibition must be determined.

Concept introductionCarnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(d)

Interpretation:

Effect of 100 µM palmitoyl CoA, 100 µM carnitine, and malonyl CoA 10µM on mutant enzyme and wild type enzyme must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(e)

Interpretation:

Role of glutamate 3 in CPTI function should be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 22, Problem 57P

Interpretation Introduction

(a)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of carnitine must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(b)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of palmitoyl CoA must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(c)

Interpretation:

The enzyme that is more sensitive to malonyl CoA inhibition must be determined.

Concept introductionCarnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(d)

Interpretation:

Effect of 100 µM palmitoyl CoA, 100 µM carnitine, and malonyl CoA 10µM on mutant enzyme and wild type enzyme must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(e)

Interpretation:

Role of glutamate 3 in CPTI function should be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 5

Chapter 22, Problem 57P

Interpretation Introduction

(a)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of carnitine must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(b)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of palmitoyl CoA must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(c)

Interpretation:

The enzyme that is more sensitive to malonyl CoA inhibition must be determined.

Concept introductionCarnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(d)

Interpretation:

Effect of 100 µM palmitoyl CoA, 100 µM carnitine, and malonyl CoA 10µM on mutant enzyme and wild type enzyme must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Interpretation Introduction

(e)

Interpretation:

Role of glutamate 3 in CPTI function should be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Answer 6

Chapter 22, Problem 57P

Interpretation Introduction

(a)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of carnitine must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Answer 7

Chapter 22, Problem 57P

Interpretation Introduction

(a)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of carnitine must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of palmitoyl CoA must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The effect of enzyme mutation on enzyme activity with varying concentrations of palmitoyl CoA must be determined.

The K_M and V_max values of wild-type and mutant enzymes must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Answer 10

Interpretation Introduction

(c)

Interpretation:

The enzyme that is more sensitive to malonyl CoA inhibition must be determined.

Concept introductionCarnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Answer 11

Interpretation Introduction

(c)

Interpretation:

The enzyme that is more sensitive to malonyl CoA inhibition must be determined.

Concept introductionCarnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Answer 12

Interpretation Introduction

(d)

Interpretation:

Effect of 100 µM palmitoyl CoA, 100 µM carnitine, and malonyl CoA 10µM on mutant enzyme and wild type enzyme must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Answer 13

Interpretation Introduction

(d)

Interpretation:

Effect of 100 µM palmitoyl CoA, 100 µM carnitine, and malonyl CoA 10µM on mutant enzyme and wild type enzyme must be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Answer 14

Interpretation Introduction

(e)

Interpretation:

Role of glutamate 3 in CPTI function should be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Answer 15

Interpretation Introduction

(e)

Interpretation:

Role of glutamate 3 in CPTI function should be determined.

Concept introduction:

Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.

Answer 16

Expert Solution & Answer

Answer 17

Expert Solution & Answer

Answer 18

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 19

Ch. 22 - Prob. 1P Ch. 22 - Prob. 2P Ch. 22 - Prob. 3P Ch. 22 - Prob. 4P Ch. 22 - Prob. 5P Ch. 22 - Prob. 6P Ch. 22 - Prob. 7P Ch. 22 - Prob. 8P Ch. 22 - Prob. 9P Ch. 22 - Prob. 10P

Solution Summary: The author explains that the effect of enzyme mutation on enzyme activity with varying concentrations of palmitoyl CoA must be determined.

Concept explainers

Want to see the full answer?

Chapter 22 Solutions