Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 22, Problem 53P
Interpretation Introduction

Interpretation:

The reaction that is common in the conversion of NADH to NADPH and gluconeogenesis should be determined along with the fate of the product formed by this reaction in gluconeogenesis.

Concept introduction:

The most important difference between NADPH and NADH is that NADPH is used during photosynthesis, and NADH is used during respiration. NADH plays an important role during oxidative phosphorylation, it acts as an electron done in this process and helps in the synthesis of ATP. One NADH molecule produces 2.5 ATP molecules.

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a) What are the differences between the Direct & Indirect Immunofluorescence Assays? (0.5 mark) b) What are the advantages of the Indirect Immunofluorescence Assays? (0.5 mark) c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality? (2 marks)
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