The purpose behind the kanamycin, plant growth hormone and carbenicillin in the media, and the action shown in media when kanamycin is left out. Introduction: T- DNA (transfer deoxyribonucleic acid) of Agrobacterium tumefaciens is used for the production of transgenic plants. T- DNA is inserted in the chromosomal DNA of the plant as a vector for production of cloned genes. The vector gene is then transformed into the bacteria. The plant is then exposed to the bacteria. A rapid cell divisionfollows the infection of Agrobacterium . The cells of the plant are treated with kanamycin and carbenicillin. This agent is used in the media to kill Agrobacterium tumefaciens .

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Chapter 22, Problem 4EQ

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The purpose behind the kanamycin, plant growth hormone and carbenicillin in the media, and the action shown in media when kanamycin is left out.

Introduction:

T- DNA (transfer deoxyribonucleic acid) of Agrobacterium tumefaciens is used for the production of transgenic plants. T- DNA is inserted in the chromosomal DNA of the plant as a vector for production of cloned genes. The vector gene is then transformed into the bacteria. The plant is then exposed to the bacteria. A rapid cell divisionfollows the infection of Agrobacterium. The cells of the plant are treated with kanamycin and carbenicillin. This agent is used in the media to kill Agrobacterium tumefaciens.

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