Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 22, Problem 3CT
Indicators are used in autoclaving to ensure that sterilization is complete. One type of chemical indicator turns color when it has reached a specific temperature; the other type turns color when it has reached a specified temperature and been exposed to steam. Which type of indicator should be used?
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Which physical or chemical agent would be the best choice for sterilizing the following items: glass pipettes, tryptic soy broth tubes, nutrient agar, antibiotic solution, interior of a biological safety cabinet, wrapped package of plastic Petri plates? Explain your choices.
In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either.
Materials:
200-ml Erlenmeyer flask Stove
500-ml Erlenmeyer flask Autoclave
10-mL graduated cylinder Analytical balance
100-ml graduated cylinder pH meter
Spatula Stirring rod
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Distilled water Petri dish
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Chapter 22 Solutions
Laboratory Experiments in Microbiology (11th Edition)
Ch. 22 - Prob. 1QCh. 22 - Prob. 2QCh. 22 - Give an example of a nonlaboratory use of each of...Ch. 22 - Define the term pasteurization. What is the...Ch. 22 - Explain why fungi and Bacillus sometimes grow...Ch. 22 - Assume that a DRT value for autoclaving a culture...Ch. 22 - Indicators are used in autoclaving to ensure that...Ch. 22 - A biological indicator used in autoclaving is a...Ch. 22 - The source of Legionella causing...
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