Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 22, Problem 3CT
Indicators are used in autoclaving to ensure that sterilization is complete. One type of chemical indicator turns color when it has reached a specific temperature; the other type turns color when it has reached a specified temperature and been exposed to steam. Which type of indicator should be used?
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Which physical or chemical agent would be the best choice for sterilizing the following items: glass pipettes, tryptic soy broth tubes, nutrient agar, antibiotic solution, interior of a biological safety cabinet, wrapped package of plastic Petri plates? Explain your choices.
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Chapter 22 Solutions
Laboratory Experiments in Microbiology (11th Edition)
Ch. 22 - Prob. 1QCh. 22 - Prob. 2QCh. 22 - Give an example of a nonlaboratory use of each of...Ch. 22 - Define the term pasteurization. What is the...Ch. 22 - Explain why fungi and Bacillus sometimes grow...Ch. 22 - Assume that a DRT value for autoclaving a culture...Ch. 22 - Indicators are used in autoclaving to ensure that...Ch. 22 - A biological indicator used in autoclaving is a...Ch. 22 - The source of Legionella causing...
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- During media preparation, you observed that the agar medium did not solidify after sterilization even after incubating it for 1 hour at room temperature. What could be the reason behind this?arrow_forwardIn the laboratory, autoclaving is required to prepare media for the growth of microorganism. What type of method of control does this involve? Dry Heat Moist Heat Chemical Radiation All of the optionsarrow_forwardFor preparation of a smear on a slide, what is the purpose of heat fixation? What problems can arise when the slide is heated in a flame?arrow_forward
- In pharmaceutical companies, Sterilization is an important step for quality assurance and approval of pharmaceutical products. Based on this concept, answer the following questions. Heat sensitive liquids need special methods for sterilization. Explain the methods applied in details.arrow_forwardGive two reasons for heating the slide after the smear is air dried?arrow_forwardOther than the application of heat, name other methods of sterilization. Define the methods and give examples of materials sterilized in each method.arrow_forward
- In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down. For "autoclaving" For Dry heat oven Can be sterilized only sterilization with either Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-ml graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-ml beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperarrow_forwardMake a list of the different types of sterilization, including the method, the principle, the materials that can be sterilized, the required temperature and time, and the required temperature and time. Method Principle Apparatus Temp/Time Materialsarrow_forwardOven sterilization at 121˚C was performed on glassware for 15 minutes only. Effect: Rationale:arrow_forward
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