Concept explainers
The source of Legionella causing hospital-acquired pneumonia has been linked to hospital water supplies. In healthcare settings, hot water coming out of the faucet should not be hotter than
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Laboratory Experiments in Microbiology (11th Edition)
- Describe methods that a cell may use to adapt to heavy metal contaminated sites. Define halophile. What is a compatible solute? What are they used for?Describe the industrial uses of Dunaliella?Describe what issues cell harvesting presents on an industrial scale.Explain what stress proteins are and how they are related to the ability of cells to live in extreme environments.arrow_forwardBelow is the colony diameter for each yeast culture grown on PDA and AcA incubated at 35°C measured at Day 7. From the data presented above, what could you observe about the growth of the yeast cultures on PDA and AcA? Cite possible reasons behind this.arrow_forwardDescribe the appearance of an S. agalactiae colony grown on blood agar. Describe how that colony would differ in appearance from a colony of S. pyogenes.arrow_forward
- The cell concentration measured in samples taken at certain intervals from a microorganism growth medium is given below. Calculate the doubling time and specific growth rate of this microorganism.arrow_forwardFor BAP, TSA, PEA, CHOC, and MAC determine if there is growth or no growth for the following: staph, strep, E. coli, and pseudomonas, and fill in the chart below BAP TSA PEA CHOC MACStaph Strep Ecoli Psu. Also answer the following using the i formation and table above 1. Which of the above media is supportive? What organisms grow on it? 2. Which of the above media is enriched? What organisms grow on it? 3. Which of the above media is selective? What organisms grow on it? 4. Which of the above media is differetial? What organisms grow on it?arrow_forwardThe solution containing bacterial spores is heated in an autoclave. When the autoclave has reached a constant temperature of 121 °C, there are still 106 spores/ml. The specific death constant for bacterial spores at 121 ° C is 11.62 min-1. How long should the heating be continued at a constant temperature so that there are 1 spores/ml left? Enter the answer in minutes to three decimal places.arrow_forward
- Nonearrow_forwardA. Antibiotics, such as gentamicin, are used in media to prevent growth of susceptible microbes. Is gentamicin media a selective, differential or enrichment media? B. Sorbitol is a sugar alcohol that all Escherichia bacteria can normally ferment into an acidic waste compound. This acidic end product is detected by including a pH indicator in the agar and noticing a color change. The strain of Escherichia that is a common food pathogen, E. coli O157:H7, however can not ferment sorbitol. It will grow similar to other E. coli, but not change the agar color. Is sorbitol in nutrient agar a selective, differential or enrichment media?arrow_forwardA culture consisting of 100 litres of nutrient medium containing 12 g l-1 of growth limiting substrate is inoculated with 10g of bacteria in a batch fermenter. The yield of biomass from substrate is 0.05 g g-1 . The maintenance coefficient is 2.0 g g-1 h -1 . The specific rate of product formation due to maintenance is 1.0 h-1 . The maximum specific growth rate of the organism is 0.3 h -1 . YPX= 7.7 and product formation is directly linked with metabolism. a) What is the time required to produce 15 g of biomass? !!!!! ANS IS 3.07 HRS!!!! STEPS on how to reach this pleasearrow_forward
- Why is the colony diameter of the yeast culture grown on potato dextrose agar is larger than those grown on acetate agar? Give and discuss the reasons for this different in growth.arrow_forwardYou have a starter culture containing 8 x 109 cells per mL, from which you take 10mL to inoculate a fresh 1 L culture. After 15 hours, the cell density of the new culture is 3 x 1012cells per mL. What are the generation time and the mean growth rate constant of theorganism in culture?arrow_forwardBelow is shown a growth curve for an E. coli culture. As indicated, the culture was incubated in the absence of a carbon source for 2 hours until Glucose and Lactose were added; Glucose was used up after 5 hours, and Lactose was used up after 8 hours. During the time-course, you take four samples, labeled A-D, after 1, 3, 7 and 9 hours, respectively, as indicated under the graph. Assuming there is cAMP present at t=0, for each sample identify whether CAP and/or the Lac Repressor would be bound to the DNA, and explain why.arrow_forward