Anatomy and Physiology: An Integrative Approach with Connect Access Card
3rd Edition
ISBN: 9781260254440
Author: Michael McKinley, Valerie O'Loughlin, Theresa Bidle
Publisher: McGraw-Hill Education
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Question
Chapter 2.2, Problem 2WDT
Summary Introduction
To predict: The specific charge of magnesium ion on the basis of its position in the periodic table.
Concept introduction: Atom is the basic unit of matter. An atom or a group of atoms having a positive charge or negative charge is called as ion. Magnesium ions are required for the production of adenosine triphosphate (ATP).
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1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Chapter 2 Solutions
Anatomy and Physiology: An Integrative Approach with Connect Access Card
Ch. 2.1 - Prob. 1LOCh. 2.1 - Prob. 2LOCh. 2.1 - Prob. 3LOCh. 2.1 - Prob. 4LOCh. 2.1 - Prob. 1WDTCh. 2.1 - What subatomic particles determine the mass of an...Ch. 2.1 - Diagram the atomic structure of chlorineatomic...Ch. 2.1 - Prob. 5LOCh. 2.1 - Prob. 6LOCh. 2.1 - Do isotopes represent the same element? Do they...
Ch. 2.1 - Prob. 7LOCh. 2.1 - Prob. 8LOCh. 2.1 - Prob. 4WDLCh. 2.2 - LEARNING OBJECTIVES
9. Define an ion.
Ch. 2.2 - LEARNING OBJECTIVES
10. List some common ions in...Ch. 2.2 - Prob. 11LOCh. 2.2 - Prob. 12LOCh. 2.2 - Prob. 2WDTCh. 2.2 - List the common cations and anions of the human...Ch. 2.2 - Prob. 6WDLCh. 2.2 - Explain how and why ions form based on the octet...Ch. 2.2 - Prob. 13LOCh. 2.2 - Prob. 14LOCh. 2.2 - Prob. 15LOCh. 2.2 - Could an ionic bond form between two cations or...Ch. 2.3 - LEARNING OBJECTIVES
16. Define a molecular...Ch. 2.3 - Prob. 17LOCh. 2.3 - Prob. 9WDLCh. 2.3 - What is an isomer?Ch. 2.3 - Prob. 18LOCh. 2.3 - Prob. 19LOCh. 2.3 - Prob. 20LOCh. 2.3 - Prob. 21LOCh. 2.3 - Explain covalent bond formation in terms of...Ch. 2.3 - Assign the partial charges between nitrogen and...Ch. 2.3 - Why are some covalent bonds nonpolar and others...Ch. 2.3 - Prob. 22LOCh. 2.3 - Prob. 23LOCh. 2.3 - WHAT DO YOU THINK?
3 Is the fatty acid portion of...Ch. 2.3 - Are O2, and CO2 nonpolar or polar molecules?Ch. 2.3 - Prob. 24LOCh. 2.3 - Prob. 25LOCh. 2.3 - What is the name of the intermolecular attraction...Ch. 2.4 - Prob. 26LOCh. 2.4 - What is the intermolecular bond that is...Ch. 2.4 - Prob. 27LOCh. 2.4 - Which property of water contributes to the need to...Ch. 2.4 - Prob. 28LOCh. 2.4 - Prob. 29LOCh. 2.4 - Prob. 30LOCh. 2.4 - How does the interaction of a nonelectrolyte and...Ch. 2.4 - How do phospholipid molecules interact with water...Ch. 2.5 - Prob. 31LOCh. 2.5 - Explain why water is neutral.Ch. 2.5 - Prob. 32LOCh. 2.5 - Which type of substance releases H+ when added to...Ch. 2.5 - Prob. 33LOCh. 2.5 - Prob. 34LOCh. 2.5 - Prob. 35LOCh. 2.5 - Prob. 4WDTCh. 2.5 - What is the general relationship of [H+] and pH?Ch. 2.5 - Why are buffers important and how do they function...Ch. 2.6 - Prob. 36LOCh. 2.6 - Prob. 37LOCh. 2.6 - Prob. 24WDLCh. 2.6 - Why is blood also considered the other two types...Ch. 2.6 - Prob. 38LOCh. 2.6 - What are four ways solution concentration may be...Ch. 2.7 - Prob. 39LOCh. 2.7 - Prob. 40LOCh. 2.7 - Prob. 41LOCh. 2.7 - Prob. 42LOCh. 2.7 - Prob. 27WDLCh. 2.7 - What functional groups may act as an acid?Ch. 2.7 - Prob. 29WDLCh. 2.7 - Prob. 43LOCh. 2.7 - Prob. 44LOCh. 2.7 - Do lipid molecules typically dissolve in water?...Ch. 2.7 - Which class of lipids forms cell membranes? What...Ch. 2.7 - Prob. 45LOCh. 2.7 - Prob. 46LOCh. 2.7 - Prob. 47LOCh. 2.7 - What is the repeating monomer of glycogen? Where...Ch. 2.7 - For each of the following, indicate if it is a...Ch. 2.7 - LEARNING OBJECTIVES
48. Describe the general...Ch. 2.7 - LEARNING OBJECTIVES
49. Describe the structure of...Ch. 2.7 - Prob. 50LOCh. 2.7 - Prob. 51LOCh. 2.7 - What is the general function of nucleic acids?Ch. 2.7 - What are the structural differences between RNA...Ch. 2.7 - Prob. 52LOCh. 2.7 - Prob. 53LOCh. 2.7 - What are the monomers of proteins and the name of...Ch. 2.8 - Prob. 37WDLCh. 2.8 - Prob. 54LOCh. 2.8 - Prob. 55LOCh. 2.8 - Prob. 56LOCh. 2.8 - Prob. 38WDLCh. 2.8 - Prob. 57LOCh. 2.8 - Prob. 58LOCh. 2.8 - Prob. 59LOCh. 2.8 - Prob. 5WDTCh. 2.8 - What distinguishes the tertiary and quaternary...Ch. 2.8 - What happens to a protein when it denatures? How...Ch. 2 - Prob. 1DYBCh. 2 - _____ 2. Substances that dissolve in water include...Ch. 2 - _____ 3. Temperature stabilization is dependent...Ch. 2 - _____ 4. All of the following are accurate about...Ch. 2 - _____ 5. Blood is a mixture that is more...Ch. 2 - Prob. 6DYBCh. 2 - _____ 7. Glucose is stored as which molecule...Ch. 2 - _____ 8. All of the following are common ions of...Ch. 2 - _____ 9. Intermolecular attractions between polar...Ch. 2 - _____ 10. When a protein permanently unfolds, it...Ch. 2 - List the common ions of the human body by name,...Ch. 2 - Describe a polar bond and a polar molecule.Ch. 2 - Diagram two water molecules and label the polar...Ch. 2 - Compare and contrast what occurs when a substance...Ch. 2 - Define the terms acid, base, PH, and buffers.Ch. 2 - Explain the units for expressing a concentration...Ch. 2 - Do You Know the Basics?
17. List the four...Ch. 2 - Prob. 18DYBCh. 2 - Describe how phospholipid molecules form the...Ch. 2 - Explain protein denaturation, including bow it...Ch. 2 - Which property of water is significant in children...Ch. 2 - Prob. 2CALCh. 2 - Prob. 3CALCh. 2 - The condition of rickets involves bones that have...Ch. 2 - The hormone insulin is a __________ composed of...Ch. 2 - Prob. 1CSLCh. 2 - The lab results from a diabetic patient show a...Ch. 2 - Prob. 3CSL
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forwardIn oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the reaction, OTMPD is electron acceptor O cytochrome c is the electron source oxygen is terminal electron acceptor OH2 produced is electron donorarrow_forward
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