BIOCHEMISTRY (HARDBACK) W/ACCESS CODE
BIOCHEMISTRY (HARDBACK) W/ACCESS CODE
6th Edition
ISBN: 9781337194204
Author: GARRETT
Publisher: CENGAGE L
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Chapter 22, Problem 17P
Interpretation Introduction

To explain:

How pentose phosphate pathway and the glycolytic pathway can combine and produce both NADPH and ATP without producing ribose-5-phosphate.

Introduction:

NADPH is produced in Pentose phosphate pathway of by oxidation but ATP is produced in glycolytic pathway by non-oxidation.

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Consider the reaction. kp S P kg What effects are produced by an enzyme on the general reaction? AG for the reaction increases. The rate constant for the reverse reaction (kr) increases. The reaction equilibrium is shifted toward the products. The concentration of the reactants is increased. The activation energy for the reaction is lowered. The formation of the transition state is promoted.
The graph displays the activities of wild-type and several mutated forms of subtilisin on a logarithmic scale. The mutations are identified as: • The first letter is the one-letter abbreviation for the amino acid being altered. • The number identifies the position of the residue in the primary structure. ⚫ The second letter is the one-letter abbreviation for the amino acid replacing the original one. • Uncat. refers to the estimated rate for the uncatalyzed reaction. Log₁(S-1) Wild type S221A H64A -5 D32A S221A H64A D32A -10 Uncat. How would the activity of a reaction catalyzed by a version of subtilisin with all three residues in the catalytic triad mutated compare to the activity of the uncatalyzed reaction? It would have more activity, because the reaction catalyzed by the triple mutant is approximately three-fold faster than the uncatalyzed reaction. It would have less activity, because the reaction catalyzed by the triple mutant is approximately 1000-fold slower than the…
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