The order of elution of aspartate before serine by pH-4 buffer through a column packed Dowex- 50 should be explained. Concept introduction: Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins. Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography. Cation-exchange chromatography separate amino acids according to their p I values, the lesser the p I -value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if the amino acids are more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

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Answer 1

Question

Chapter 21.5, Problem 18P

(a)

Interpretation Introduction

Interpretation:

The order of elution of aspartate before serine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if the amino acids are more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

(b)

Interpretation Introduction

Interpretation:

The order of elution of serine before alanine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values and polarities, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if an amino acid is more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

(c)

Interpretation Introduction

Interpretation:

The order of elution of valine before leucine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values and polarities, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if an amino acid is more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

(d)

Interpretation Introduction

Interpretation:

The order of elution of tyrosine before phenylalanine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values and polarities, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if an amino acid is more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

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Answer 2

Question

Chapter 21.5, Problem 18P

(a)

Interpretation Introduction

Interpretation:

The order of elution of aspartate before serine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if the amino acids are more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

(b)

Interpretation Introduction

Interpretation:

The order of elution of serine before alanine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values and polarities, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if an amino acid is more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

(c)

Interpretation Introduction

Interpretation:

The order of elution of valine before leucine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values and polarities, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if an amino acid is more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

(d)

Interpretation Introduction

Interpretation:

The order of elution of tyrosine before phenylalanine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values and polarities, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if an amino acid is more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

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Answer 3

Question

Chapter 21.5, Problem 18P

(a)

Interpretation Introduction

Interpretation:

The order of elution of aspartate before serine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if the amino acids are more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

(b)

Interpretation Introduction

Interpretation:

The order of elution of serine before alanine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values and polarities, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if an amino acid is more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

(c)

Interpretation Introduction

Interpretation:

The order of elution of valine before leucine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values and polarities, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if an amino acid is more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

(d)

Interpretation Introduction

Interpretation:

The order of elution of tyrosine before phenylalanine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values and polarities, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if an amino acid is more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

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Answer 4

Question

Chapter 21.5, Problem 18P

(a)

Interpretation Introduction

Interpretation:

The order of elution of aspartate before serine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if the amino acids are more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

(b)

Interpretation Introduction

Interpretation:

The order of elution of serine before alanine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values and polarities, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if an amino acid is more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

(c)

Interpretation Introduction

Interpretation:

The order of elution of valine before leucine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values and polarities, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if an amino acid is more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

(d)

Interpretation Introduction

Interpretation:

The order of elution of tyrosine before phenylalanine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values and polarities, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if an amino acid is more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

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Answer 5

Chapter 21.5, Problem 18P

(a)

Interpretation Introduction

Interpretation:

The order of elution of aspartate before serine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if the amino acids are more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

(b)

Interpretation Introduction

Interpretation:

The order of elution of serine before alanine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values and polarities, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if an amino acid is more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

(c)

Interpretation Introduction

Interpretation:

The order of elution of valine before leucine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values and polarities, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if an amino acid is more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

(d)

Interpretation Introduction

Interpretation:

The order of elution of tyrosine before phenylalanine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values and polarities, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if an amino acid is more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

Answer 6

Chapter 21.5, Problem 18P

(a)

Interpretation Introduction

Interpretation:

The order of elution of aspartate before serine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if the amino acids are more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

Answer 7

Chapter 21.5, Problem 18P

(a)

Interpretation Introduction

Interpretation:

The order of elution of aspartate before serine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if the amino acids are more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

Answer 8

(b)

Interpretation Introduction

Interpretation:

The order of elution of serine before alanine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values and polarities, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if an amino acid is more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

Answer 9

(b)

Interpretation Introduction

Interpretation:

The order of elution of serine before alanine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values and polarities, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if an amino acid is more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

Answer 10

(c)

Interpretation Introduction

Interpretation:

The order of elution of valine before leucine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values and polarities, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if an amino acid is more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

Answer 11

(c)

Interpretation Introduction

Interpretation:

The order of elution of valine before leucine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values and polarities, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if an amino acid is more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

Answer 12

(d)

Interpretation Introduction

Interpretation:

The order of elution of tyrosine before phenylalanine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values and polarities, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if an amino acid is more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

Answer 13

(d)

Interpretation Introduction

Interpretation:

The order of elution of tyrosine before phenylalanine by pH-4 buffer through a column packed Dowex-50 should be explained.

Concept introduction:

Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.

Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.

Cation-exchange chromatography separate amino acids according to their pI values and polarities, the lesser the pI-value of an amino acid as compared to given pH have the high concentration of negative charged ions. The negative charged ions elute (release from chromatogram) easily from the Cation-exchange chromatogram. And if an amino acid is more polar, then it will also elute (release from chromatogram) easily by the non-polar resins (Dowex-50).

Answer 14

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Answer 15

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Answer 16

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Answer 17

Ch. 21.1 - a. Explain why, when the imidazole ring of...Ch. 21.2 - a. Which isomer(R)-alanine or (S)-alanineis...Ch. 21.2 - Prob. 4P Ch. 21.3 - Prob. 5P Ch. 21.3 - Prob. 6P Ch. 21.3 - Draw the predominant form for glutamate in a...Ch. 21.3 - a. Why is the pKa of the glutamate side chain...Ch. 21.4 - Calculate the pI of each of the following amino...Ch. 21.4 - a. Which amino acid has the lowest pI value? b....Ch. 21.4 - Prob. 12P

Solution Summary: The author explains the order of elution of aspartate before serine by pH-4 buffer through a column packed Dowex-50.

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