Chapter 21.5, Problem 17P

Interpretation Introduction

Interpretation:

Reason for the increasing higher $\text{pH}$ of buffer solutions to elute the column that generate the chromatogram has to be explained.

Concept introduction:

Amino acid analyzer is used to separate mixture of amino acids on the basis of their overall charge by using cation-exchange resin.

Elute is the process of take-off or wash out the each amino acid from the chromatogram of amino acid analyzer.