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(a) Interpretation:
The other half of a restriction site should be drawn if the one-half is as follows:
Concept Introduction:
The restriction site also known as the restriction sequence is an enzyme that splits DNA into fragments at a recognized site inside a molecule. The restriction site's other part is given and the type II cleavage site with the blunt-ended duplex DNA is shown with heavy arrows. The type II cleavage sites yielding staggered cut is given by light arrows. For the type I restriction endonuclease recognition, the duplex cut is at 1 kilobase away and identical copies of DNA sequence interval is 4096 kilobase pairs as the sequence is 6 base longs with 4 possibility positioned
(b) Interpretation:
Type II cleavage sites producing blunt-ended duplex DNA should be identified using heavy arrows.
Concept Introduction:
The restriction site also known as the restriction sequence is an enzyme that splits DNA into fragments at a recognized site inside a molecule. The restriction site's other part is given and the type II cleavage site with the blunt-ended duplex DNA is shown with heavy arrows. The type II cleavage sites yielding staggered cut is given by light arrows. For the type I restriction endonuclease recognition, the duplex cut is at 1 kilobase away and identical copies of DNA sequence interval is 4096 kilobase pairs as the sequence is 6 base longs with 4 possibility positioned nucleotides.
(c) Interpretation:
Type II cleavage sites producing staggered cuts converted to recombinant DNA molecules by DNA ligase without enzyme involvement should be identified using light arrows.
Concept Introduction:
The restriction site also known as the restriction sequence is an enzyme that splits DNA into fragments at a recognized site inside a molecule. The restriction site's other part is given and the type II cleavage site with the blunt-ended duplex DNA is shown with heavy arrows. The type II cleavage sites yielding staggered cut is given by light arrows. For the type I restriction endonuclease recognition, the duplex cut is at 1 kilobase away and identical copies of DNA sequence interval is 4096 kilobase pairs as the sequence is 6 base longs with 4 possibility positioned nucleotides.
(d) Interpretation:
The place where the duplex be cut when the recognition site for type I restriction endonuclease should be identified.
Concept Introduction:
The restriction site also known as the restriction sequence is an enzyme that splits DNA into fragments at a recognized site inside a molecule. The restriction site's other part is given and the type II cleavage site with the blunt-ended duplex DNA is shown with heavy arrows. The type II cleavage sites yielding staggered cut is given by light arrows. For the type I restriction endonuclease recognition, the duplex cut is at 1 kilobase away and identical copies of DNA sequence interval is 4096 kilobase pairs as the sequence is 6 base longs with 4 possibility positioned nucleotides.
(e) Interpretation:
The average interval of identical copies of the given sequence in DNA which is random should be identified in kilobase pairs.
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Chapter 21 Solutions
Biochemistry: Concepts and Connections
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