PRIN.OF HUMAN ANATOMY (LL)
PRIN.OF HUMAN ANATOMY (LL)
15th Edition
ISBN: 9781119662761
Author: Tortora
Publisher: WILEY
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Chapter 21, Problem 39CP
Summary Introduction

To review:

The sites of production of blood cells outside and inside the embryo.

Introduction:

Blood is a fluid connective tissue that circulates in the blood vessels of organisms. In placental mammals, an embryo is an unborn offspring that is in the process of development. After the eighth week of fertilization, the embryo is termed as a fetus.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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