The results of western blot of a gene on mutation in varying conditions like: 1. Normal gene from a plant. 2. Gene without promoter. 3. Heterozygous mutation in which one gene is normal and other is short due to introduction of a stop codon at 112 position. 4. Homozygous mutation in which stop codon is inserted at 112 base pair (bp). 5. Homozygous mutation in which phenylalanine is changed to leucine. Introduction: In western blotting, the fragments of polypeptides that are produced within the cell are detected by using the antibodies as probes. The proteins from cell are purified and are loaded in the electrophoresis. These proteins then are observed by autoradiography or fluorescence.

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Answer 1

Question

Chapter 21, Problem 25EQ

Summary Introduction

To analyze:

The results of western blot of a gene on mutation in varying conditions like:

1. Normal gene from a plant.

2. Gene without promoter.

3. Heterozygous mutation in which one gene is normal and other is short due to introduction of a stop codon at 112 position.

4. Homozygous mutation in which stop codon is inserted at 112 base pair (bp).

5. Homozygous mutation in which phenylalanine is changed to leucine.

Introduction:

In western blotting, the fragments of polypeptides that are produced within the cell are detected by using the antibodies as probes. The proteins from cell are purified and are loaded in the electrophoresis. These proteins then are observed by autoradiography or fluorescence.

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Answer 2

Question

Chapter 21, Problem 25EQ

Summary Introduction

To analyze:

The results of western blot of a gene on mutation in varying conditions like:

1. Normal gene from a plant.

2. Gene without promoter.

3. Heterozygous mutation in which one gene is normal and other is short due to introduction of a stop codon at 112 position.

4. Homozygous mutation in which stop codon is inserted at 112 base pair (bp).

5. Homozygous mutation in which phenylalanine is changed to leucine.

Introduction:

In western blotting, the fragments of polypeptides that are produced within the cell are detected by using the antibodies as probes. The proteins from cell are purified and are loaded in the electrophoresis. These proteins then are observed by autoradiography or fluorescence.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 3

Question

Chapter 21, Problem 25EQ

Summary Introduction

To analyze:

The results of western blot of a gene on mutation in varying conditions like:

1. Normal gene from a plant.

2. Gene without promoter.

3. Heterozygous mutation in which one gene is normal and other is short due to introduction of a stop codon at 112 position.

4. Homozygous mutation in which stop codon is inserted at 112 base pair (bp).

5. Homozygous mutation in which phenylalanine is changed to leucine.

Introduction:

In western blotting, the fragments of polypeptides that are produced within the cell are detected by using the antibodies as probes. The proteins from cell are purified and are loaded in the electrophoresis. These proteins then are observed by autoradiography or fluorescence.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 21, Problem 25EQ

Summary Introduction

To analyze:

The results of western blot of a gene on mutation in varying conditions like:

1. Normal gene from a plant.

2. Gene without promoter.

3. Heterozygous mutation in which one gene is normal and other is short due to introduction of a stop codon at 112 position.

4. Homozygous mutation in which stop codon is inserted at 112 base pair (bp).

5. Homozygous mutation in which phenylalanine is changed to leucine.

Introduction:

In western blotting, the fragments of polypeptides that are produced within the cell are detected by using the antibodies as probes. The proteins from cell are purified and are loaded in the electrophoresis. These proteins then are observed by autoradiography or fluorescence.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 5

Chapter 21, Problem 25EQ

Summary Introduction

To analyze:

The results of western blot of a gene on mutation in varying conditions like:

1. Normal gene from a plant.

2. Gene without promoter.

3. Heterozygous mutation in which one gene is normal and other is short due to introduction of a stop codon at 112 position.

4. Homozygous mutation in which stop codon is inserted at 112 base pair (bp).

5. Homozygous mutation in which phenylalanine is changed to leucine.

Introduction:

In western blotting, the fragments of polypeptides that are produced within the cell are detected by using the antibodies as probes. The proteins from cell are purified and are loaded in the electrophoresis. These proteins then are observed by autoradiography or fluorescence.

Answer 6

Chapter 21, Problem 25EQ

Summary Introduction

To analyze:

The results of western blot of a gene on mutation in varying conditions like:

1. Normal gene from a plant.

2. Gene without promoter.

3. Heterozygous mutation in which one gene is normal and other is short due to introduction of a stop codon at 112 position.

4. Homozygous mutation in which stop codon is inserted at 112 base pair (bp).

5. Homozygous mutation in which phenylalanine is changed to leucine.

Introduction:

In western blotting, the fragments of polypeptides that are produced within the cell are detected by using the antibodies as probes. The proteins from cell are purified and are loaded in the electrophoresis. These proteins then are observed by autoradiography or fluorescence.

Answer 7

Chapter 21, Problem 25EQ

Summary Introduction

To analyze:

The results of western blot of a gene on mutation in varying conditions like:

1. Normal gene from a plant.

2. Gene without promoter.

3. Heterozygous mutation in which one gene is normal and other is short due to introduction of a stop codon at 112 position.

4. Homozygous mutation in which stop codon is inserted at 112 base pair (bp).

5. Homozygous mutation in which phenylalanine is changed to leucine.

Introduction:

In western blotting, the fragments of polypeptides that are produced within the cell are detected by using the antibodies as probes. The proteins from cell are purified and are loaded in the electrophoresis. These proteins then are observed by autoradiography or fluorescence.

Answer 8

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Answer 9

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Answer 10

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Answer 11

Ch. 21.1 - 1. Which of the following may be used as a vector...Ch. 21.1 - The restriction enzymes used in gene-cloning...Ch. 21.1 - 3. Which is the proper order of the following...Ch. 21.1 - 4. The function of reverse transcriptase is...Ch. 21.1 - A collection of recombinant vectors that carry...Ch. 21.2 - Prob. 1COMQ Ch. 21.2 - Prob. 2COMQ Ch. 21.2 - 3. During real-time PCR, the synthesis of PCR...Ch. 21.3 - When a dideoxyribonucleotide is incorporated into...Ch. 21.4 - 1. The purpose of site-directed mutagenesis and...

Solution Summary: The author analyzes the results of western blotting of a gene on mutation in varying conditions.

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