BIOCHEMISTRY
BIOCHEMISTRY
8th Edition
ISBN: 9781319296186
Author: BERG
Publisher: MAC HIGHER
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Chapter 21, Problem 24P
Interpretation Introduction

Introduction:

Glycogen synthase b enzyme regulation should be explained.

Concept introduction:

Glycogen synthesis is allostericallyregulated.The phosphorylated glycogen synthase b enzyme gets activated in the presence of glucose-6-phosphate, which is its allosteric activator.

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You’ve isolated a protein and determined that the Native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD.  The enzyme was found to be 0.829% NAD (by weight). What further can be said regarding the structure of the polypeptide?
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