Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
7th Edition
ISBN: 9781464126116
Author: David L. Nelson, Michael M. Cox
Publisher: W. H. Freeman
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Chapter 21, Problem 1P

(a)

Summary Introduction

To determine: Using the knowledge of fatty acid biosynthesis, given experimental observation: Addition of uniformly labeled [14C] acetyl-CoA to a soluble liver fraction yields palmitate uniformly labeled with [14C].

Introduction:

Fatty acid is the common pathway which occurs in the cytoplasm of the cell. Fatty acid synthesis, acetyl-CoA and NADPH produce fatty acids in the presence of enzyme fatty acid synthases.

(a)

Expert Solution
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Explanation of Solution

Explanation:

The 16 carbon molecules of palmate are derived from 8 acetyl groups of 8 acetyl-CoA molecules. The addition of [14C] acetyl-CoA will produce malonyl-CoA which is labeled at [C-1] and [C-2], followed by production of labeled palmitate. The acetyl-CoA is needed to load the acyl carrier protein, in the presence of acetyl-CoA carboxylase, acetyl-CoA, and CO2will produce malonyl-CoA.

(b)

Summary Introduction

To determine: Using the knowledge of fatty acid biosynthesis, given experimental observation: b. Addition of a trace of uniformly labeled [14C] acetyl-CoA in the presence of an excess of unlabeled malonyl-CoA to a soluble liver fraction yields palmitate labeled with [14C] only in C-15 and C-16  .

Introduction:

Fatty acid is the common pathway which occurs in the cytoplasm of the cell. Fatty acid synthesis, acetyl-CoA and NADPH produce fatty acids in the presence of enzyme fatty acid synthases.

(b)

Expert Solution
Check Mark

Explanation of Solution

Explanation:

The metabolic pool of malonyl-CoA is the main resource of all palmitate carbons except carbon-16 and carbon-15. The trace amount of [14C] acetyl-CoA cannot label the pool of malonyl-CoA , hence in the presence of unlabeled  malonyl-CoA in excess amount addition trace of uniformly labeled[14C] acetyl-CoA, the acyl- carrier protein is loaded with labeled[14C] acetyl-CoA and produced palmitate instead of malonyl-CoA , which is labeled as [C-15] and [C-16].

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