Campbell Biology, Books a la Carte Edition (11th Edition)
Campbell Biology, Books a la Carte Edition (11th Edition)
11th Edition
ISBN: 9780134154121
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece
Publisher: PEARSON
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Chapter 20.1, Problem 2CC

DRAW IT Ø One Strand of a DNA molecule has the follow- ing sequence:

5'-CTTGACGATCGTTACCG-3'

Draw the other Strand. Will PvuI (see question 1) cut this molecule? If so, draw the products.

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Draw a rough sketch of the control and experimental data using a normal dose/response curve plot: i.e. % of total bound ligand vs. concentration. Indicate Kd and Bmax on this sketch. You don’t need to use exact numbers, but the relative proportions should be close
Name something that could be happening to glutamate transporters that could have this effect on glutamate transport (i.e. the difference in glutamate transporters between control and experimental conditions). (Bonus: if you can also guess what the experimental condition is).
a. For the control condition, calculate Kd. Include units and show your work. b. For the control condition, calculate Bmax. Include units and show your work.  c. For the experimental condition, calculate Kd. Include units and show your work. For the experimental condition, calculate Bmax. Include units and show your work.
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