Lehninger Principles of Biochemistry (Instructor's)
Lehninger Principles of Biochemistry (Instructor's)
7th Edition
ISBN: 9781319117689
Author: nelson
Publisher: MAC HIGHER
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Chapter 20, Problem 34P
Summary Introduction

To determine: The factor that determines which of these pathways (citric acid cycle or glyoxylate cycle) isocitrate will enter.

Introduction:

Cellular respiration is a process of release of energy by the catabolism of glucose molecules. In the cellular respiration, the cells use the oxygen molecules for breakdown of glucose and releases adenosine triphosphate (ATP) that act as energy storing molecules.

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Please analze the gel electrophoresis column of the VRK1 kinase (MW: 39.71 kDa).  Lane 1:  buffer Lane 2 : Ladder Lane 3: Lysate Lane 4: Flowthrough Lane 5: Wash Lanes 6-8: E1, E2, E3 Lane 9: Dialyzed VRK1  Lane 10: LDH
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You have isolated a protein and determined that the native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD. Describe the architecture of the polypeptide component of this enzyme.
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