
To determine: The consequences if the transgene integrates randomly into the coding region, or the regulatory region, of an endogenous gene.
Introduction: Recombinant DNA (rDNA) technology has made it possible to directly manipulate genes in vivo using different techniques to learn more about gene function in living organisms. The modification of gene of interest and its reinsertion into an organism of the same species or a gene from an individual of one species is inserted into another species; these both approaches will result in the transgenic organism.
To determine: The reason due to which the random integration of the transgene into the coding region, or the regulatory region will complicate the genetic analysis of the transgene.
Introduction: Transgenics explains the technique of inserting foreign DNA into a host organism’s genome. In multicellular organisms, this technique can be performed by experimental manipulation of gametes or early embryos. It is very important that the vector containing transgene is incorporated into the target gene; otherwise, there will be dangerous consequences.

Want to see the full answer?
Check out a sample textbook solution
Chapter 20 Solutions
Concepts of Genetics Plus Mastering Genetics with Pearson eText -- Access Card Package (12th Edition) (What's New in Genetics)
- What is the concept "calories consumed must equal calories burned" in regrads to nutrition?arrow_forwardYou intend to insert patched dominant negative DNA into the left half of the neural tube of a chick. 1) Which side of the neural tube would you put the positive electrode to ensure that the DNA ends up on the left side? 2) What would be the internal (within the embryo) control for this experiment? 3) How can you be sure that the electroporation method itself is not impacting the embryo? 4) What would you do to ensure that the electroporation is working? How can you tell?arrow_forwardDescribe a method to document the diffusion path and gradient of Sonic Hedgehog through the chicken embryo. If modifying the protein, what is one thing you have to consider in regards to maintaining the protein’s function?arrow_forward
- The following table is from Kumar et. al. Highly Selective Dopamine D3 Receptor (DR) Antagonists and Partial Agonists Based on Eticlopride and the D3R Crystal Structure: New Leads for Opioid Dependence Treatment. J. Med Chem 2016.arrow_forwardThe following figure is from Caterina et al. The capsaicin receptor: a heat activated ion channel in the pain pathway. Nature, 1997. Black boxes indicate capsaicin, white circles indicate resinferatoxin. You are a chef in a fancy new science-themed restaurant. You have a recipe that calls for 1 teaspoon of resinferatoxin, but you feel uncomfortable serving foods with "toxins" in them. How much capsaicin could you substitute instead?arrow_forwardWhat protein is necessary for packaging acetylcholine into synaptic vesicles?arrow_forward
- 1. Match each vocabulary term to its best descriptor A. affinity B. efficacy C. inert D. mimic E. how drugs move through body F. how drugs bind Kd Bmax Agonist Antagonist Pharmacokinetics Pharmacodynamicsarrow_forward50 mg dose of a drug is given orally to a patient. The bioavailability of the drug is 0.2. What is the volume of distribution of the drug if the plasma concentration is 1 mg/L? Be sure to provide units.arrow_forwardDetermine Kd and Bmax from the following Scatchard plot. Make sure to include units.arrow_forward
- Choose a catecholamine neurotransmitter and describe/draw the components of the synapse important for its signaling including synthesis, packaging into vesicles, receptors, transporters/degradative enzymes. Describe 2 drugs that can act on this system.arrow_forwardThe following figure is from Caterina et al. The capsaicin receptor: a heat activated ion channel in the pain pathway. Nature, 1997. Black boxes indicate capsaicin, white circles indicate resinferatoxin. a) Which has a higher potency? b) Which is has a higher efficacy? c) What is the approximate Kd of capsaicin in uM? (you can round to the nearest power of 10)arrow_forwardWhat is the rate-limiting-step for serotonin synthesis?arrow_forward
- Human Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage LearningConcepts of BiologyBiologyISBN:9781938168116Author:Samantha Fowler, Rebecca Roush, James WisePublisher:OpenStax CollegeBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
- Case Studies In Health Information ManagementBiologyISBN:9781337676908Author:SCHNERINGPublisher:CengageEssentials Health Info Management Principles/Prac...Health & NutritionISBN:9780357191651Author:BowiePublisher:CengageBiology (MindTap Course List)BiologyISBN:9781337392938Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. BergPublisher:Cengage Learning



