To assume: You performed a DNA sequencing reaction using the Sanger method. Despite performing all the steps correctly, the sequencing gel had many numbers of duplicate bands in terms of their length in the other lanes. What might have happened? Introduction: Sanger sequencing is a method of chain termination which is used for determining the DNA nucleotide sequences. It provides every possible various length of DNA fragments terminated with the tagged nucleotides at the 3’ end. These products are then separated by gel electrophoresis.

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Chapter 20, Problem 23PDQ

Summary Introduction

To assume:

You performed a DNA sequencing reaction using the Sanger method. Despite performing all the steps correctly, the sequencing gel had many numbers of duplicate bands in terms of their length in the other lanes. What might have happened?

Introduction:

Sanger sequencing is a method of chain termination which is used for determining the DNA nucleotide sequences. It provides every possible various length of DNA fragments terminated with the tagged nucleotides at the 3’ end. These products are then separated by gel electrophoresis.

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