Vector is a key material that is used in the process of gene cloning. It acts as a carrier of the DNA segment that has to be cloned. When this vector is introduced into a living cell, it replicates with the DNA it carries and produces many identical copies of the inserted gene.

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Answer 1

Question

Chapter 20, Problem 1TY

Summary Introduction

Introduction: Vector is a key material that is used in the process of gene cloning. It acts as a carrier of the DNA segment that has to be cloned. When this vector is introduced into a living cell, it replicates with the DNA it carries and produces many identical copies of the inserted gene.

Expert Solution & Answer

Answer to Problem 1TY

Correct answer: The vectors used to clone genes were derived originally from plasmids or viruses. Hence, the correct answer is option e.

Explanation of Solution

Reason for correct answer:

The vectors commonly used in the gene cloning experiments are derived from plasmids or viruses. The plasmids contain unique sites into which the gene of interest can be easily inserted. Viruses can also be used as vectors to carry the pieces of DNA. This is because when a gene of interest is inserted into a viral vector, the gene gets replicated along with the replication of the viral DNA.

Option e. is given as “b and c only”.

Plasmids are small, circular pieces of DNA that are naturally found in many strains of bacteria. The plasmids exist independently of the bacterial chromosome. The plasmids can be genetically engineered for the effective use in cloning experiments. The viral vectors infect living cells and propagate themselves by taking control of the host cell’s metabolic machinery. Hence, the correct answer is option e.

Reasons for incorrect answer:

Option a. is given as, “proteins”.

The vectors are not derived from proteins. Hence, option a. is incorrect.

Option b. is given as, “plasmids”.

The vectors are derived not only from plasmids but also from the viruses. Hence, option b. is incomplete.

Option c. is given as, “viruses”.

The vectors are derived not only from viruses but also from plasmids. Hence, option c. is incomplete.

Option d. is given as, “all of the above”.

The vectors are derived from plasmid or viruses, but not from proteins. Hence, option d. is incorrect.

Hence, the options a., b., c., and d. are incorrect.

Conclusion

Hence, the vectors used to clone the genes are derived originally from viruses or plasmids.

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Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…

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Answer 2

Question

Chapter 20, Problem 1TY

Summary Introduction

Introduction: Vector is a key material that is used in the process of gene cloning. It acts as a carrier of the DNA segment that has to be cloned. When this vector is introduced into a living cell, it replicates with the DNA it carries and produces many identical copies of the inserted gene.

Expert Solution & Answer

Answer to Problem 1TY

Correct answer: The vectors used to clone genes were derived originally from plasmids or viruses. Hence, the correct answer is option e.

Explanation of Solution

Reason for correct answer:

The vectors commonly used in the gene cloning experiments are derived from plasmids or viruses. The plasmids contain unique sites into which the gene of interest can be easily inserted. Viruses can also be used as vectors to carry the pieces of DNA. This is because when a gene of interest is inserted into a viral vector, the gene gets replicated along with the replication of the viral DNA.

Option e. is given as “b and c only”.

Plasmids are small, circular pieces of DNA that are naturally found in many strains of bacteria. The plasmids exist independently of the bacterial chromosome. The plasmids can be genetically engineered for the effective use in cloning experiments. The viral vectors infect living cells and propagate themselves by taking control of the host cell’s metabolic machinery. Hence, the correct answer is option e.

Reasons for incorrect answer:

Option a. is given as, “proteins”.

The vectors are not derived from proteins. Hence, option a. is incorrect.

Option b. is given as, “plasmids”.

The vectors are derived not only from plasmids but also from the viruses. Hence, option b. is incomplete.

Option c. is given as, “viruses”.

The vectors are derived not only from viruses but also from plasmids. Hence, option c. is incomplete.

Option d. is given as, “all of the above”.

The vectors are derived from plasmid or viruses, but not from proteins. Hence, option d. is incorrect.

Hence, the options a., b., c., and d. are incorrect.

Conclusion

Hence, the vectors used to clone the genes are derived originally from viruses or plasmids.

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Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 3

Question

Chapter 20, Problem 1TY

Summary Introduction

Introduction: Vector is a key material that is used in the process of gene cloning. It acts as a carrier of the DNA segment that has to be cloned. When this vector is introduced into a living cell, it replicates with the DNA it carries and produces many identical copies of the inserted gene.

Expert Solution & Answer

Answer to Problem 1TY

Correct answer: The vectors used to clone genes were derived originally from plasmids or viruses. Hence, the correct answer is option e.

Explanation of Solution

Reason for correct answer:

The vectors commonly used in the gene cloning experiments are derived from plasmids or viruses. The plasmids contain unique sites into which the gene of interest can be easily inserted. Viruses can also be used as vectors to carry the pieces of DNA. This is because when a gene of interest is inserted into a viral vector, the gene gets replicated along with the replication of the viral DNA.

Option e. is given as “b and c only”.

Plasmids are small, circular pieces of DNA that are naturally found in many strains of bacteria. The plasmids exist independently of the bacterial chromosome. The plasmids can be genetically engineered for the effective use in cloning experiments. The viral vectors infect living cells and propagate themselves by taking control of the host cell’s metabolic machinery. Hence, the correct answer is option e.

Reasons for incorrect answer:

Option a. is given as, “proteins”.

The vectors are not derived from proteins. Hence, option a. is incorrect.

Option b. is given as, “plasmids”.

The vectors are derived not only from plasmids but also from the viruses. Hence, option b. is incomplete.

Option c. is given as, “viruses”.

The vectors are derived not only from viruses but also from plasmids. Hence, option c. is incomplete.

Option d. is given as, “all of the above”.

The vectors are derived from plasmid or viruses, but not from proteins. Hence, option d. is incorrect.

Hence, the options a., b., c., and d. are incorrect.

Conclusion

Hence, the vectors used to clone the genes are derived originally from viruses or plasmids.

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Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Question

Chapter 20, Problem 1TY

Summary Introduction

Introduction: Vector is a key material that is used in the process of gene cloning. It acts as a carrier of the DNA segment that has to be cloned. When this vector is introduced into a living cell, it replicates with the DNA it carries and produces many identical copies of the inserted gene.

Expert Solution & Answer

Answer to Problem 1TY

Correct answer: The vectors used to clone genes were derived originally from plasmids or viruses. Hence, the correct answer is option e.

Explanation of Solution

Reason for correct answer:

The vectors commonly used in the gene cloning experiments are derived from plasmids or viruses. The plasmids contain unique sites into which the gene of interest can be easily inserted. Viruses can also be used as vectors to carry the pieces of DNA. This is because when a gene of interest is inserted into a viral vector, the gene gets replicated along with the replication of the viral DNA.

Option e. is given as “b and c only”.

Plasmids are small, circular pieces of DNA that are naturally found in many strains of bacteria. The plasmids exist independently of the bacterial chromosome. The plasmids can be genetically engineered for the effective use in cloning experiments. The viral vectors infect living cells and propagate themselves by taking control of the host cell’s metabolic machinery. Hence, the correct answer is option e.

Reasons for incorrect answer:

Option a. is given as, “proteins”.

The vectors are not derived from proteins. Hence, option a. is incorrect.

Option b. is given as, “plasmids”.

The vectors are derived not only from plasmids but also from the viruses. Hence, option b. is incomplete.

Option c. is given as, “viruses”.

The vectors are derived not only from viruses but also from plasmids. Hence, option c. is incomplete.

Option d. is given as, “all of the above”.

The vectors are derived from plasmid or viruses, but not from proteins. Hence, option d. is incorrect.

Hence, the options a., b., c., and d. are incorrect.

Conclusion

Hence, the vectors used to clone the genes are derived originally from viruses or plasmids.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Chapter 20, Problem 1TY

Summary Introduction

Introduction: Vector is a key material that is used in the process of gene cloning. It acts as a carrier of the DNA segment that has to be cloned. When this vector is introduced into a living cell, it replicates with the DNA it carries and produces many identical copies of the inserted gene.

Expert Solution & Answer

Answer to Problem 1TY

Correct answer: The vectors used to clone genes were derived originally from plasmids or viruses. Hence, the correct answer is option e.

Explanation of Solution

Reason for correct answer:

The vectors commonly used in the gene cloning experiments are derived from plasmids or viruses. The plasmids contain unique sites into which the gene of interest can be easily inserted. Viruses can also be used as vectors to carry the pieces of DNA. This is because when a gene of interest is inserted into a viral vector, the gene gets replicated along with the replication of the viral DNA.

Option e. is given as “b and c only”.

Plasmids are small, circular pieces of DNA that are naturally found in many strains of bacteria. The plasmids exist independently of the bacterial chromosome. The plasmids can be genetically engineered for the effective use in cloning experiments. The viral vectors infect living cells and propagate themselves by taking control of the host cell’s metabolic machinery. Hence, the correct answer is option e.

Reasons for incorrect answer:

Option a. is given as, “proteins”.

The vectors are not derived from proteins. Hence, option a. is incorrect.

Option b. is given as, “plasmids”.

The vectors are derived not only from plasmids but also from the viruses. Hence, option b. is incomplete.

Option c. is given as, “viruses”.

The vectors are derived not only from viruses but also from plasmids. Hence, option c. is incomplete.

Option d. is given as, “all of the above”.

The vectors are derived from plasmid or viruses, but not from proteins. Hence, option d. is incorrect.

Hence, the options a., b., c., and d. are incorrect.

Conclusion

Hence, the vectors used to clone the genes are derived originally from viruses or plasmids.

Answer 6

Chapter 20, Problem 1TY

Summary Introduction

Introduction: Vector is a key material that is used in the process of gene cloning. It acts as a carrier of the DNA segment that has to be cloned. When this vector is introduced into a living cell, it replicates with the DNA it carries and produces many identical copies of the inserted gene.

Expert Solution & Answer

Answer to Problem 1TY

Correct answer: The vectors used to clone genes were derived originally from plasmids or viruses. Hence, the correct answer is option e.

Explanation of Solution

Reason for correct answer:

The vectors commonly used in the gene cloning experiments are derived from plasmids or viruses. The plasmids contain unique sites into which the gene of interest can be easily inserted. Viruses can also be used as vectors to carry the pieces of DNA. This is because when a gene of interest is inserted into a viral vector, the gene gets replicated along with the replication of the viral DNA.

Option e. is given as “b and c only”.

Plasmids are small, circular pieces of DNA that are naturally found in many strains of bacteria. The plasmids exist independently of the bacterial chromosome. The plasmids can be genetically engineered for the effective use in cloning experiments. The viral vectors infect living cells and propagate themselves by taking control of the host cell’s metabolic machinery. Hence, the correct answer is option e.

Reasons for incorrect answer:

Option a. is given as, “proteins”.

The vectors are not derived from proteins. Hence, option a. is incorrect.

Option b. is given as, “plasmids”.

The vectors are derived not only from plasmids but also from the viruses. Hence, option b. is incomplete.

Option c. is given as, “viruses”.

The vectors are derived not only from viruses but also from plasmids. Hence, option c. is incomplete.

Option d. is given as, “all of the above”.

The vectors are derived from plasmid or viruses, but not from proteins. Hence, option d. is incorrect.

Hence, the options a., b., c., and d. are incorrect.

Conclusion

Hence, the vectors used to clone the genes are derived originally from viruses or plasmids.

Answer 7

Chapter 20, Problem 1TY

Summary Introduction

Introduction: Vector is a key material that is used in the process of gene cloning. It acts as a carrier of the DNA segment that has to be cloned. When this vector is introduced into a living cell, it replicates with the DNA it carries and produces many identical copies of the inserted gene.

Answer 8

Expert Solution & Answer

Answer to Problem 1TY

Correct answer: The vectors used to clone genes were derived originally from plasmids or viruses. Hence, the correct answer is option e.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Explanation of Solution

Reason for correct answer:

The vectors commonly used in the gene cloning experiments are derived from plasmids or viruses. The plasmids contain unique sites into which the gene of interest can be easily inserted. Viruses can also be used as vectors to carry the pieces of DNA. This is because when a gene of interest is inserted into a viral vector, the gene gets replicated along with the replication of the viral DNA.

Option e. is given as “b and c only”.

Plasmids are small, circular pieces of DNA that are naturally found in many strains of bacteria. The plasmids exist independently of the bacterial chromosome. The plasmids can be genetically engineered for the effective use in cloning experiments. The viral vectors infect living cells and propagate themselves by taking control of the host cell’s metabolic machinery. Hence, the correct answer is option e.

Reasons for incorrect answer:

Option a. is given as, “proteins”.

The vectors are not derived from proteins. Hence, option a. is incorrect.

Option b. is given as, “plasmids”.

The vectors are derived not only from plasmids but also from the viruses. Hence, option b. is incomplete.

Option c. is given as, “viruses”.

The vectors are derived not only from viruses but also from plasmids. Hence, option c. is incomplete.

Option d. is given as, “all of the above”.

The vectors are derived from plasmid or viruses, but not from proteins. Hence, option d. is incorrect.

Hence, the options a., b., c., and d. are incorrect.

Answer 12

Conclusion

Hence, the vectors used to clone the genes are derived originally from viruses or plasmids.

Answer 13

Ch. 20.1 - Prob. 1CC Ch. 20.1 - Prob. 1BC Ch. 20.1 - Prob. 2CC Ch. 20.1 - Prob. 3CC Ch. 20.1 - Prob. 4CC Ch. 20.1 - Prob. 2BC Ch. 20.2 - Prob. 1CC Ch. 20.2 - Prob. 2CC Ch. 20.2 - Prob. 3CC Ch. 20.3 - Prob. 1CC

Vector is a key material that is used in the process of gene cloning. It acts as a carrier of the DNA segment that has to be cloned. When this vector is introduced into a living cell, it replicates with the DNA it carries and produces many identical copies of the inserted gene.

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Introduction: Vector is a key material that is used in the process of gene cloning. It acts as a carrier of the DNA segment that has to be cloned. When this vector is introduced into a living cell, it replicates with the DNA it carries and produces many identical copies of the inserted gene.

Answer to Problem 1TY

Explanation of Solution

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