EBK BIOLOGY
4th Edition
ISBN: 8220102797376
Author: BROOKER
Publisher: YUZU
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 20, Problem 5TY
Why is Taq polymerase used in PCR rather than other DNA polymerases?
- a. Taq polymerase is a synthetic enzyme that produces DNA strands at a faster rate than natural polymerases.
- b. Taq polymerase is a heat-stable form of DNA polymerase that can function after exposure to the high temperatures necessary for PCR.
- c. Taq polymerase is easier to isolate than other DNA polymerases.
- d. Taq polymerase is the DNA polymerase commonly produced by most eukaryotic cells.
- e. All of the above are correct.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Would it be possible to use human polymerase for the PCR reaction?
a.
No, because human polymerase does not have the ability to withstand the high temperatures required for the PCR reaction to occur.
b.
No, because human polymerase cannot be extracted from cells to use in a lab setting.
c.
Yes, because we are using human DNA as the template DNA.
d.
Yes, because human polymerase can add bases to a template strand without a primer.
Choose the one answer that fits best. Which statement regarding PCR is NOT correct (videos)?
a.
PCR requires a copy of RNA that serves as a template
b.
Taq polymerase adds nucleotides to the primers and creates a complementary strand of DNA
c.
Annealing requires cooler temperatures than denaturation
d.
Repeated cycles of denaturation, annealing and extending DNA strands creates many identical copies of DNA
e.
PCR is a quick way of using minute quantities of DNA to create millions of copies
PCR is a technique used to synthesize DNA fragments. Select all the reagents needed for PCR to occur.
A. DNA template
B. Thermo stable DNA polymerase
C. Two primers
D. Type I endonucleases
Chapter 20 Solutions
EBK BIOLOGY
Ch. 20.1 - Prob. 1CCCh. 20.1 - Prob. 1BCCh. 20.1 - Prob. 2CCCh. 20.1 - Prob. 3CCCh. 20.1 - Prob. 4CCCh. 20.1 - Prob. 2BCCh. 20.2 - Prob. 1CCCh. 20.2 - Prob. 2CCCh. 20.2 - Prob. 3CCCh. 20.3 - Prob. 1CC
Ch. 20.3 - Prob. 2CCCh. 20.3 - Prob. 3CCCh. 20.3 - Prob. 4CCCh. 20.3 - Prob. 1EQCh. 20.3 - Prob. 2EQCh. 20.3 - Prob. 3EQCh. 20 - Prob. 1TYCh. 20 - Prob. 2TYCh. 20 - DNA ligase is needed in a cloning experiment a. to...Ch. 20 - Prob. 4TYCh. 20 - Why is Taq polymerase used in PCR rather than...Ch. 20 - Lets suppose you want to clone a gene that has...Ch. 20 - Prob. 7TYCh. 20 - Prob. 8TYCh. 20 - Prob. 9TYCh. 20 - Prob. 10TYCh. 20 - Prob. 1CQCh. 20 - Prob. 2CQCh. 20 - Prob. 3CQCh. 20 - Identify and discuss three important advances that...Ch. 20 - Prob. 2COQ
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- What is the purpose of the low temperature step in the PCR reaction? a. To allow DNA polymerase to synthesize new DNA in the 3' to 5' direction b. To permanently deactivate DNA polymerase c. To allow primers to anneal to DNA templates d. To allow DNA polymerase to synthesize new DNA in the 5' to 3' directionarrow_forwardPlace the following steps in order to outline how enzymes are involved with proofreading newly formed DNA molecules. 1. DNA polymerase does not detect damaged DNA. 2. Ligase connects the free end of the new DNA with the old DNA. 3. DNA polymerase replaces the damaged DNA with the correct nucleotide. 4. Exonuclease cuts the damaged DNA strand in order to remove the damaged section.arrow_forwardMatch the following terms with their definitions and label each component of the PCR mixture in the diagram (use the letters A-D):I. DNA polymeraseII. PrimersIII. NucleotidesIV. Genomic DNA template A. DNA that contains the target sequence that will be replicated using PCR.B. An enzyme that copies the DNA sequence.C. A mixture of 4 nucleotides (A,G,C, and T) that will be polymerized into the replicated DNA sequence.D. A short DNA sequence that allows the enzyme to bind and initiate polymerization.arrow_forward
- For DNA amplification using PCR to occur, which of the following are needed? A. DNA primers B. thermo stable DNA polymerase C. Helicase D. Choices A and Barrow_forwardDNA fragments that are 500 bp, 1000 bp, and 2000 bp in length are separated by gel electrophoresis. Which fragment will migrate farthest in the gel? a. The 2000-bp fragment b. The 1000-bp fragment c. The 500-bp fragment d. All will migrate equal distances.arrow_forwardWhat is DNA polymerase? a.An enzyme that carries out DNA replication b.Short, single strand of DNA that base-pairs with a specific DNA sequence c.An enzyme that corrects mutations that arise during the replication of DNA d.An enzyme that seals any gaps that remain between bases of replicating strands of DNAarrow_forward
- Match the terms associated with the polymerase chain reaction with their correct descriptions. Refers to the fact that DNA molecules get longer the more of them there are in the reaction. A. В. Heat the sample to a high temperature (usually 94°C) to separate all DNA strands from each other. Denaturation C. Incubate the reaction at the optimal temperature for the primers to base-pair with each other. Annealing. D. Incubate at a low enough temperature (usually-55°C) so that primers base-pair with their complementary sequence. Extension. Add a chaotropic agent that destabilizes hydrogen bonding. E. Amplification. F. Incubate the sample at a temperature that is optimal for thermostable Taq DNA polymerase (usually -72°C). G. Happens after repeated cycles of the temperature change regimen. Refers to the quadrupling of the target DNA sequence in every cycle of the temperature regimen. Н.arrow_forwardPredict the effect on the PCR reaction if any of the following circumstances arose: i) there are no primers in the reaction, i) there are no dNTPs in the reaction, iii) there is no Taq polymerase in the reaction, A PCR would not commence. B. PCR would proceed normally. C. PCR would cease after a few cycles. D. Non-specific DNA amplification will occur.arrow_forwardDuring a PCR reaction, all the following events occur except: A. Hybridization B. Binding of a 3’OH group in the active site of DNA polymerase C. Addition of nucleotide monomers on the 3’ end of the template DNA D. DNA denaturation E. Semi conservative replicationarrow_forward
- Which one is correct?arrow_forwardChoose the one answer that fits best. Which statement regarding Molecular Biology is NOT correct (videos)? a. Taq Polymerase was isolated from an organism found in Yellowstone Park b. Restriction enzymes leave sticky ends c. DNA sequencing allows us to read DNA sequences d. Restriction enzymes cut DNA at specific sites e. EcoRI and HindII are commonly used polymerasesarrow_forwardWhich of the following best describes the process of DNA seqencing. a. DNA is seperated on a gel and the different bands are labled with flouroscent nucleotides and scanned with a laser. b. A laser is used to flurorescently label the nucleotides present with in the DNA , the DNA is run on a gel and then the DNA is droken into fragments c. Nucleotides are scanned with a laser and incrprorated into the DNA that has been seperated on a gel and then DNA is amplified with PCR. d. fragments of DNA are produced in a reaction that lables them with any of four different fluroscent dyes and the fragmented then are run on a gel and scanned with laser e. DNA is broken down into its constituents nucleotides and the nucleotides are then run on a gel and purified with a laserarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
Molecular Techniques: Basic Concepts; Author: Dr. A's Clinical Lab Videos;https://www.youtube.com/watch?v=7HFHZy8h6z0;License: Standard Youtube License