The reason behind the nonworking of two primers which have been purchased for the Polymerase chain reactions. The way by which one can salvage the experiment of PCR (Polymerase chain reaction). Introduction: Polymerase chain reaction (PCR) is an advanced technique by which millions of copies of the desired segment of the DNA (Deoxyribose nucleic acid ) can be made with the help of the thermocycler. The PCR consisted of three steps: the denaturation, the annealing, and the extension. In the denaturation step, the unwinding of the double-stranded DNA takes place. During the annealing process, the short strand of bases which is called primer gets attached to the single-stranded DNA. In the extension step, the extension of the primer takes place. A thermostable DNA polymerase enzyme is required for carrying out the reactions. Please refer the table given in the question for sequences of both the primers and the template strand.

Question

Want to see the full answer?

Answer 1

Question

Chapter 20, Problem 16TYPSS

Summary Introduction

To review:

The reason behind the nonworking of two primers which have been purchased for the Polymerase chain reactions.

The way by which one can salvage the experiment of PCR (Polymerase chain reaction).

Introduction:

Polymerase chain reaction (PCR) is an advanced technique by which millions of copies of the desired segment of the DNA (Deoxyribose nucleic acid) can be made with the help of the thermocycler. The PCR consisted of three steps: the denaturation, the annealing, and the extension. In the denaturation step, the unwinding of the double-stranded DNA takes place. During the annealing process, the short strand of bases which is called primer gets attached to the single-stranded DNA. In the extension step, the extension of the primer takes place. A thermostable DNA polymerase enzyme is required for carrying out the reactions. Please refer the table given in the question for sequences of both the primers and the template strand.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb? Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.

Design a grafting experiment to determine if limb mesoderm determines forelimb / hindlimb identity. Include the experiment, a control, and an interpretation in your answer.

The Snapdragon is a popular garden flower that comes in a variety of colours, including red, yellow, and orange. The genotypes and associated phenotypes for some of these flowers are as follows: aabb: yellow AABB, AABb, AaBb, and AaBB: red AAbb and Aabb: orange aaBB: yellow aaBb: ? Based on this information, what would the phenotype of a Snapdragon with the genotype aaBb be and why? Question 21 options: orange because A is epistatic to B yellow because A is epistatic to B red because B is epistatic to A orange because B is epistatic to A red because A is epistatic to B yellow because B is epistatic to A