Biological Science
Biological Science
5th Edition
ISBN: 9780321743671
Author: Scott Freeman
Publisher: PEARSON
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Chapter 21, Problem 1TYK
Summary Introduction

Introduction:

The way, in which the nucleotide sequence in a nucleic acid molecule is divided into a set of non-overlapping nucleotide triplets is called reading frame. An ORF (open reading frame) is a reading frame that can be transcribed into an RNA (ribonucleic acid) and then, translated to form a protein.

Expert Solution & Answer
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Answer to Problem 1TYK

Correct answer:

The ORF in bacteria is the section of DNA or deoxyribonucleic acid that codes for a protein as it has a start as well as a stop codon that flanks hundreds of nucleotides.

Explanation of Solution

Explanation/justification for the correct answer:

Option (c) is given that the ORF is that section of DNA, which have a start codon and a stop codon that lie either before and after the nucleotide stretch. The ORFs are a part of reading frames in molecular biology. It is a stretch of codons, which is found in genes. They are generally continuous in nature.

This stretch of codon has a start codon and a stop codon. In an ORF, the start codon indicates the start of the translation. The end of the transcription of the gene is the site where the codon stops. It is generally located after the ORF. Hence, option (c) is correct.

Explanation for the incorrect answers:

Option (a) is given that the ORFs are the genes, whose functions are already known. The genes whose functions are already known are the protein-coding genes. They are represented by expressed sequence tag. The ORFs, which are found using programming tools are compared with the sequence of the already known genes from another species through a computer program. So, it is an incorrect option.

Option (b) is given that the ORF is a DNA segment, which codes for a protein because it is similar to complementary DNA or cDNA. A cDNA is a DNA that is synthesized from a single strand of RNA. It is used in the cloning process of the gene in prokaryotes. Whereas, the ORFs, are the segments that are found in the DNA through the process of transcription. So, it is an incorrect option.

Option (d) is given that the ORFs are any member of the gene family. However, the ORFs are the DNA segments, which are found in the gene with the help of the computer programs. It is a part of DNA that transcribes and then translates to form the proteins. So, it is an incorrect option.

Hence, options, (a), (b), and (d) are incorrect.

Conclusion

Therefore, it can be said that the ORF in bacteria is the DNA sections that is thought to code for a protein. They comprise of a start codon and a stop code that flanks the hundreds of nucleotides.

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